Domain Reconstruction and Lysis Activity of Phage Lysin

doi:10.7668/hbnxb.2017.06.020

Abstract

Abstract: In order to get wide host-range phage lysin,the cell-binding domains of Staphylococcus aureus Ply187 CHAP and SH3b (lysin K) were synthesized based on the sequence,according to the specificity and structure of the phage lysin.The cell-binding domain was amplified from Listeria monocytogenes phage lysin LysZ5.All the domain were reconstructed and the lysis function were analyzed.From the SDS-PAGE,reconstruction lysin Ply187KS-Z5,containing two cell-binding domains,showed the specific protein band at 58 kDa.Clear zone observed after Lysin Ply187KS-Z5 added on the plate of both S.aureus and L.monocytogenes. Lysin Ply187-KS could reduce number of S. aureus to 2.16 log in 1 hour,but the reconstruction lysin Ply187KS-Z5 was able to inhibit the growth of both S.aureus and L.monocytogenes.Therefore,domain reconstruction should be a new approach to develop wide-host-range lysin.

Key words: Lysin, Staphylococcus aureus, Listeria monocytogenes

CLC Number:

S432.4

ZHANG Hui, ZHOU Yan, BAO Hongduo, YANG Zhenquan, ZHU Ruyi, ZHOU Chenlu, ZHANG Lili, WANG Ran. Domain Reconstruction and Lysis Activity of Phage Lysin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 134-138. doi: 10.7668/hbnxb.2017.06.020.

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