Cloning and Functional Analysis of VcMYB Promoter from Vaccinium corymbosum

doi:10.7668/hbnxb.2017.02.003

Abstract

Abstract: In order to study the regulatory role of VcMYB promoter during the transcription,the promoter sequence of the VcMYB gene,regulated the proanthocyanidin synthesis genes from highbush blueberry,was cloned by the methods of FPNI-PCR.The 768 bp promoter sequence was analyzed by PLACE and Plant CARE.The analysis revealed that the promoter sequence contained CAAT-box,TATA-box and some cis-acting element such as light responsive element,cis-acting element involved in low-temperature,MeJA,defense and stress responsiveness.In order to study the function of VcMYB promoter,a promoter-reporter vector VcMYBpro::GUS was constructed,and introduced into Arabidopisis thaliana by Agrobacterium-mediated method.The result of GUS histochemical staining showed that GUS reporter gene expression was driven by VcMYB promoter in transgenic plants,and GUS enzyme activity of transgenic plants has increased after different treatments,including ABA (Abscisic acid),4℃ low temperature,LED light and continuous light.These results indicated that VcMYB promoter was regulated by ABA,low temperature and light.

Key words: Vaccinium corymbosum, VcMYB, Promoter, GUS gene, Proanthocyanidin

CLC Number:

Q78
S668.03

SHI Wenjun, TENG Ke, CHEN Lu, HOU Zhixia, SU Shuchai. Cloning and Functional Analysis of VcMYB Promoter from Vaccinium corymbosum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 14-20. doi: 10.7668/hbnxb.2017.02.003.

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