The Cloning and Expression Analysis of Transcription Factor GhSNAC3 Promoter in Cotton

doi:10.7668/hbnxb.2016.05.006

Abstract

Abstract: In order to analyze the structure and function of the promoter of cotton NAC transcription factor gene GhSNAC3,the promoter fragment was cloned and identified from Lumianyan 32 in Gossypium hirsutum L..The cis-acting elements in the promoter sequence was predicted and analyzed by using the online database of Plant CARE,PLACER and BDGP.A plant expression vector consisting of GhSNAC3 and its promoter was constructed and transformed into Nicotiana tabacum L.to investigate the transcription activities of the promoter under different stress treatments.The results showed that besides the basic cis-acting elements such as CAAT-box,there existed several MeJA,GA and ABA-responsive elements as well as multiple light-responsive elements and stress-induced controlling elements in the promoter.Under salt stress GhSNAC3 had a high level of expression in the roots of transgenic tobacco plants,while the expression level was low in the stems and leaves,suggesting that the gene GhSNAC3 might played an important role in stress responses in cotton,and its promoter might be tissue-specific and salt-inducible.The results provide the basis for the further study of cotton NAC signal pathway as well as salt tolerance improvement through genetic engineering in the future.

Key words: Cotton, NAC, Promoter, Salt-induced

CLC Number:

Q78
S562.03

MA Junjun, LIU Zhanji, LI Fei, WANG Liguo, FU Mingchuan, ZHU Xinxia, LIU Renzhong. The Cloning and Expression Analysis of Transcription Factor GhSNAC3 Promoter in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 35-43. doi: 10.7668/hbnxb.2016.05.006.

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