Transgenic Rice with High Expression of C4-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions

doi:10.7668/hbnxb.2015.04.017

Abstract

Abstract: The objective of this study was to reveal transgenic rice with high expression of maize C₄-PEPC photosynthetic performance under low nitrogen conditions,two rice genotypes including C₄-PEPC transgenic rice (PC)and untransgenic Kitaake rice(WT) in the pot experiments were chosen to measure their photosynthesis characteristics,SPAD values,PEPC,Rubisco,NR and GS activities of flag leaves at different flowering stage under different nitrogen applications(normal nitrogen 300 kg/ha,low nitrogen 65 kg/ha) and their yield component were investigated after harvest.The results showed that the net photosynthetic rate of PC compared with WT were increased by 13.10% (P <0.05) and 29.29% (P <0.05),respectively in the 14,28 d after flowering under low nitrogen treatment.At the same time,under low concentrations of nitrogen,relative to untransformed wild type rice,activity of Rubisco carboxylase of PC were increased by 67.86% and 52.63% (P <0.05) in the 14,28 d after flowering respectively,while activity of nitrate reductase of PC were increased 79.49% (P <0.05) and 17.96%.Meanwhile,activity of glutamine synthetase of PC was increased 28.48% (P <0.05) only in the 28 days after flowering comparing with WT.But we did not find significant differences in the yield of PC and WT under low nitrogen conditions.Therefore,PC maintain high net photosynthetic rate by inducing the activity of carbon and nitrogen key enzyme improvement under low nitrogen conditions.

Key words: High expression of maize C₄-PEPC transgenic rice, Low nitrogen, Photosynthesis

CLC Number:

TANG Yu-ting, LI Xia, LU Wei, WEI Xiao-dong. Transgenic Rice with High Expression of C₄-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 95-100. doi: 10.7668/hbnxb.2015.04.017.

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Transgenic Rice with High Expression of C₄-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions

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