Characterization of the Promoter of a Rice rTGA4 Like Transcription Factor

doi:10.3969/j.issn.1000-7091.2013.04.001

Abstract

Abstract: The family of TGA transcription factors cooperates with NPR1 to play a very important role in dis- ease defence． A 1 995 bp sequence of the 5'UTR of a new TGA transcription factor rTGA4 was isolated from rice mutant HX- 3 genomic DNA and named pTGA， which shows 94% similarity to the genomic sequence of nipponbare． Sequence analysis of the promoter by PLACE and Plant CARE showed that the cloned fragment contained such some basic transcription element TATA and CAAT- Box， and some putative cis- elements， such as abscisic acid respon- seive， ethylene responsive， MeJA responsive， gibberellin- responsive and pathogen responsive elements． Then an ex- pression vector was constructed by connecting the pTGA with pCXGUS- T /A and it was transformed to Arabidopsis thaliana by Agrobacterium- mediated method． The transgenic plants were analyzed by PCR amplification and GUS staining． We found that GUS gene was mainly expressed in shoot tip of seedling and did not detect GUS activity in other parts， but the specific expression was lost during mature period． So we consider that the pTGA is a tissue spe- cific promoter which is regulated by growth stage． Characterization of the promoter of the rTGA4 lays a foundation for cloning the resistant gene in HX- 3 and its further application．

Key words: Rice, TGA transcription factors, Promoter, Arabidopsis, GUS stainin

CLC Number:

LIAN Zi-yi, YANG Yu-wen, CHEN Tian-zi, ZHANG Bao-long, LIU Ai-min. Characterization of the Promoter of a Rice rTGA4 Like Transcription Factor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 1-6. doi: 10.3969/j.issn.1000-7091.2013.04.001.

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