Genetic Variation Analysis in Maize Mutants from AS-9 Inbread Line Based on SSR Marker

doi:10.3969/j.issn.1000-7091.2013.03.018

Abstract

Abstract: Analysis of genetic diversity in maize germplasm is of great importance to conserving and utilizing these valuable chemical mutagenic resources． In this research， the genetic differences between mutants ( M1＆M4) and the corresponding orginal material( AS-9) was studied based on SSR markers with selected 54 polymorphism SSR primers． The results showed that the seed germination rate，bud length and root number of M1 ＆M4 were higher than AS-9 in most cases． In two generations maize materials，54 polymorphism primers can be amplified，accounting for 79． 41% of the total． Relatived toⅠCK ， the average value of genetic similarity coefficient of M1 was 0．364 7．With the certered genetic similarity coefficient of - 0.12 by UPGMA， the maize inbred line materials were divided into two groups: the orginal material(ⅠCK) and mutants． Relatived to ⅣCK， the average value of genetic similarity coefficient of M4 was 0．434 6． With the cemtered genetic similarity coefficient of - 0.09， the maize materials were classified into two groups: ⅣCK and mutants． Principal component analysis and clustering results were similar． The results showed there were distinct variation between orginal material AS-9 and induced material， indicating chemical mutagen had produced genetic variations in maize inbred lines on large scale．

Key words: Maize, Inbred lines, Chemical mutagenesis, SSR markers, Genetic variation

CLC Number:

S335.3

Li Hongying, Lu Cunfu, Lan Xiaozhong, Yang Fengjiao, Jin Deshan, Qiao Pei, Lu Xiao, Yü Meng, Chen Yuzhen. Genetic Variation Analysis in Maize Mutants from AS-9 Inbread Line Based on SSR Marker[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 92-101. doi: 10.3969/j.issn.1000-7091.2013.03.018.

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