Abstract: According to the study on histological damage induced by the different doses of copper sulfate， this paper can provide reference for the study of animal reproductive toxicity and reproduction． Firstly，LD50 was 55 mg /kg ( 95% CI， 35． 02 to 155) which was determined by Up-and-Down Procedure( UDP) ． 40 rats were randomly separated into control group( normal saline) ， low-dose copper sulfate group，middle-dose copper sulfate group，high-dose copper sulfate group． The rats were exposed to copper sulfate by gavage every day and the treatment was lasted for 28 days．Body weight of rats were recorded during experiment， organ coefficient of tissues and pathology changes of testis were observed after poisoning． The results showed that， in comparison with control group，body weight increasing rate was differently decreased on body weight in each poisoning group． The high-dose group experienced negative growth in the first and second weeks， and were -3． 86%，-0． 62% respectively． Obvious pathological changes through light microscope were observed in testis tissues，seminiferous tubules showed degeneration in varying degrees in poisoning groups． The number of all the spermatogenic cells in each grade in seminiferous tubules and spermatozoa were decreased or absent obviously． Testicular pathologic injury was more serious with the increase of concentration． To sum up， copper sulfate can induce the apoptosis of spermatogenic cells， and can significantly inhibit the proliferation of spermatogonia． The effect of reproductive toxic on testicular tissue are stronger as doses increased

Key words: Copper sulfate, Rat, Organ coefficient, Testis, Pathological changes

CLC Number: 

• S852.3