Abstract: The single factor test and orthogonal-design experiment were applied to screen optimal fermentation technology such as medium composition and fermentation conditions for Bacillus amyloliquefaciens HN with water purification,and the fermentation parameters in fermentor of 100 L was scaled up successfully. The results showed that the strain HN was easy to cultivate in a medium with 2.0g/L peptone,3.0g/L beef extract,7.0g/L soluble starch,6.0g/L NaCl and the optimum fermentation conditions were initial culture medium pH 7. 0,inoculation rate 3%(V/V),100mL/250mL medium volume at 37℃ for 24-30 h. Under this condition,strain HN reached loga-rithmic phase at 9 h,came to stable period after cultured at 37℃ for 24 h and the bacteria concentration reached 35. 6×108cfu/mL and the spore concentration was up to 30.4×108cfu/mL in 250mL triangular flask. The valida-tion test in 100 L fermentor result indicated that bacteria number and spore concentration reached 37. 0×108 cfu/mL and 33. 6×108cfu/mL,respectively after fermented at 37℃ for 26 h.

Key words: Bacillus amyloliquefaciens, Fermentation parameters, Optimization

CLC Number: 

• S154. 3