Colonization Observation and Control Efficiency Test against Cabbage Wilt Disease of GFP Tagged Bacillus subtilisKct99

doi:10.3969/j.issn.1000-7091.2012.06.012

Abstract

Abstract: Marked strain gfp-Kct99 with luminous phenotype fluorescent was obtained by transmitting plasmid pGFP4412 containing green fluorescent protein gene． The heredity test showed that the stability of plasmid pGFP4412 in engineering strains was 89%; The confrontation culture and pot experiment proved that marked strain gfp-Kct99 maintained the original antagonistic activity to cabbage fusarium oxysporium; The control efficiency of the marked and wild strains were 87． 7% and 90． 2% against cabbage wilt disease respectively when they were vaccinated 5 d ahead of pathogenic bacteria，no significant differences between the two strains，but significantly superior to that of the two treatments when the antagonistic and Pathogenic bacteria were vaccinated simultaneously or the antagonistic bacteria was vaccinated 5 d later． The results showed that the activity of the marked strain gfp-Kct99 against cabbage blight was not influenced by GFP marking． Observing by fluorescence microscope showed that the marked strain was able to colonize in the root skin of the cabbage to stop the invasion of the pathogen．

Key words: Bacillus subtilis, Green fluorescent protein( GFP) , Colonize tracking, Cabbage wilt disease, Biological control

CLC Number:

Q343.1

TIAN Zhao-feng, LIU Wei-cheng, DONG Dan, LI Yong-dan, ZHANG Tao-tao, LIU De-wen. Colonization Observation and Control Efficiency Test against Cabbage Wilt Disease of GFP Tagged Bacillus subtilisKct99[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 53-57. doi: 10.3969/j.issn.1000-7091.2012.06.012.

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