Expression Analysis of Disease Resistance-related Genes in Rice Plant Induced by Paenibacillus kribbensis PS04

doi:10.7668/hbnxb.20190312

Abstract

Abstract: Paenibacillus kribbensis PS04 is a high-efficiency bacterium for the biocontrol of rice sheath blight. In order to investigate the expression profiles of various disease resistance-related genes,rice leaves were harvested at 3,6,9,12,24 h after 24 h sprayed with fermentation liquid of PS04 strain. Real-time PCR analysis were performed to monitor the expression levels of rice disease resistance-related genes after treatment with PS04 strain. The fermentation liquid of PS04 strain could induce the expression of defense related genes(OsPR1, OsPR2, OsPR3, OsPR4, OsPR5, OsPR10, OsPR16 and NPR1 ),salicylic acid pathway genes(PAL and OsNAC4 ),jasmonic acid and ethylene pathways genes(AOS2, LOX and EIN2 ). The expression of NPR1 and LOX genes reached a maximum level at 3 h and then decreased. The expression of OsPR3, OsPR4, OsPR5, OsPR16 and PAL genes reached a maximum level at 6 h and then decreased. The expression of OsPR1 and OsNAC4 genes reached a maximum level at 9 h and then decreased. The expression of OsPR10 genes reached a maximum level at 12 h and then decreased. The expression of OsPR2 and EIN2 genes reached a maximum level at 24 h. The expression of AOS2 gene was inhibited,and the expression reached the highest level at 6 h and then decreased. The result of this study showed that the PS04 strain could induce both systemic acquired resistance and induced systemic resistance in rice plant.

Key words: Rice, Paenibacillus, Resistance-related genes, Inducing resistance, Biological control

CLC Number:

S432.2+3

CHENG Yan, QU Weiyin, ZHENG Wenbo, YANG Mei, LIAO Meide, ZHOU Erxun, SHU Canwei. Expression Analysis of Disease Resistance-related Genes in Rice Plant Induced by Paenibacillus kribbensis PS04[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 203-209. doi: 10.7668/hbnxb.20190312.

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