Differentiation of Umbilical Cord Mesenchymal Stem Cells （UCMSCs） Derived from Chicken into Isletβ-cells in vitro

doi:10.3969/j.issn.1000-7091.2012.04.023

Abstract

Abstract: The aim was to study on the biological characteristics of chicken UCMSCs by isolating culture in vitro. UCMSCs was isolated from umbilical cord of 11 -15 day-old chicken embryos. The suitable culture medium for UCMSCs was L-DMEM. Then the morphology and the growing proliferation condition and the differentiation po- tential in vitro of the chicken UCMSCs were observed. The results showed that Chicken UCMSCs can be cultured in vitro and have been passed to 30 generation. The UCMSCs expressed CD29,CD44 and CDT1,results displayed that the UCMSCs had the differentiation potential to isletβ-cells. UCMSCs from chicken can proliferate rapidly in vitro and be induced to isletβ-cells.

Key words: Chicken, UCMSCs, Isolation and in vitro culture Identification, Differentiation potential

CLC Number:

WU Shuang, GUAN Wei-jun, MA Yue-hui. Differentiation of Umbilical Cord Mesenchymal Stem Cells （UCMSCs） Derived from Chicken into Isletβ-cells in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 123-129. doi: 10.3969/j.issn.1000-7091.2012.04.023.

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References

[1] Karahuseyinoglu S, Kocaefe C, Balci D, et al. Functional structure of adipocytes differentiated from human umbili- cal cord stroma-derived stern cells[J]. Stern Cells,2008, 26(3) :682 -691.

[2] Karahuseyinoglu S, Cinar O, Kilic E,et al. Biology of stem cells in human umbilical cord stroma:In situ and in vitro surveys [J]. Stem Cells, 2007,25 : 319 - 331.

[3] Lumelsky N, Blondel O, Laeng P, et al. Differentiation of embryonic stem cells to insulin-secreting structures simi- lar to pancreatic islets [J]. Science, 2001,292 ( 5520 ) : 1389 - 1394.

[4] Vaca P, Mart n F, Vegara-Meseguer J M, et al. Induction of differentiation of embryonic stem cells into insulin-se- creting ceils by fetal soluble factors [J]. Stem Cells, 2006,24 ( 2 ) : 258 - 265.

[5] Segev H, Fishman B, Ziskind A, et al. Differentiation of human embryonic stem cells into insulin-producing clus- ters[J]. Stem Cells,2004,22(3 ) :265 - 274.

[6] Moriscot C, Fraipont F D, Richard M J,et al. Human bone marrow mesenchymal stem cells can express insulin and key transcription factors of the endocrine pancreas devel- opmental pathway upon genetic and/or microenvironmen- tal manipulation in vitro [J]. Stem Cells, 2005,23 ( 4 ) : 594 - 603.

[7] Seaberg R M,Smukler S R,Kieffer T J,et al. Clonal iden- tification of muhipotent precursors from adult mouse pan- creas that generate neural and pancreatic lineages [J]. Nat Biotech,2004,22 : 1115 - 1124.

[8] Tai M H,Karl O L,Burra V M,et al. Characterization of gap junctional intercellular communication in immortalized human pancreatic ductal epithelial cells with stem cell characteristics [J]. Pancreas, 2003,26 ( 1 ) : 18 - 26.

[9] Linning, Katrina D, Tai M H, et al. Redox-mediated en- richment of self-renewing adult human pancreatic cells that possess endocrine differentiation [J]. Potential Pan- creas,2004,29(3 ) :64 - 67.

[10] Yang L J, Li S W, Hatch H, et al. In vitro transdifferenti- ation of adult hepatic stem cells into pancreatic endo- crine hormone-producing cells [J]. Proc Natl Acad Sci USA,2002,99:8078 - 8083.

[11] Hori Y C, Gu X Y, Xie X D, et al. Differentiation of in- sulin-producing cells from human neural progenitor ceils [J]. PLoS Med,2005,2:347 -356.

[12] Ende N,Chen R F, Reddi Alluru S. Effect of human um- bilical cord blood cells on glycemia and insulitis in type 1 diabetic mice [J]. Biochem Biophys Res Commun, 2004,325:665 - 669.

[13] Ende N, Chen R F, Reddi A S. Transplantation of human umbilical cord blood cells improves glycemia and glo- merular hypertrophy in type 2 diabetic mice [J]. Bio- chem Biophys. Res Commun,2004,321:168 - 171.

[14] Petsa A, Gargani S, Felesakis A,et al. Effectiveness of protocol for the isolation of Wharton's Jelly stem cells in large-scale applications[J]. In Vitro Cell Dev Biol -Ani- mal,2009,45:573 - 576.

[15] Chao K C,Chao K F,Fu Y S,et al. Islet-like clusters de- rived from mesenchymal stem cells in Wharton's Jelly of the human umbilical cord for transplantation to control type 1 diabetes[J]. PLoS One,2008 (1) :1451.

[16] Takahash I T, Kalka C, Masuda H,et al. Ischemia and cytokine induced mobiliazatio of bone marrow derived endothelial progeitor cells for neovasculation [J]. Nat Med, 1999,8:434 -438.

[17] Zulewski H,Abraham E J,Gerlach M J,et al. Multipo- tential nestin-positive stem cells isolated from adult pan- creatic endocrine, exocrine and hepatic phenotypes [J]. Diabetes,2001,50:521 - 533.

[18] Otonkoski T,Beattie G M,Mally M I,et al. Nicotinamide is a potent inducer of endocrine differentiation in cul- tured human fetal pancreatic cells [J]. J Clin Invest, 1993.92 : 1459 - 1466.

[19] Roche E,Buteau J,Aniento I,et al. Induction by glucose of genes coding for glycolytic enzymes in a pancreatic beta-cell line(INS-1 ) [J]. J Boil Chem,1997,272(5) : 3091 - 3098.

[20] Ramiya V K, Maraist M, Arfors E,et al. Reversal of insu- lin dependent diabetes using islets generated in vitro from pancreatic stem ceils [J]. Nat Med, 2000,6 ( 3 ) : 278 - 282.

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