Oral Inoculation with O157:H7(ΔhlyΔstxΔtoxB) Leads to Decreased Shedding in Goats after Challenge with Escherichia coli O157:H7

doi:10.3969/j.issn.1000-7091.2012.03.024

ACTA AGRICULTURAE BOREALI-SINICA ›› 2012, Vol. 27 ›› Issue (3): 123-125. doi: 10.3969/j.issn.1000-7091.2012.03.024

Special Issue: Animal husbandry；

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Oral Inoculation with O157:H7(ΔhlyΔstxΔtoxB) Leads to Decreased Shedding in Goats after Challenge with Escherichia coli O157:H7

ZHANG Xue-han, HE Kong-wang, YU Zheng-yu, MAO Ai-hua

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Engineering Research of Veterinary Bio-products of Agricultural Ministry, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China

Received:2012-04-08 Published:2012-06-28

Abstract

Abstract: Clarify whether oral inoculation with O157:H7(ΔhlyΔstxΔtoxB) leads to decreased shedding in?goats after challenge with Escherichia coli O157:H7. Three months old goats were chosen to orally inoculate EHEC?O157:H7(ΔhlyΔstxΔtoxB)at 0 day and inoculate parent EHEC O157:H7 at 7 days and 14 days，respectively.?EHEC O157:H7 of shedding in feces were detected by plate cultivation. The goats challenged with EHEC O157:H7?at 7 days did not shed bacteria in their feces on the 4th day，and the goats at 14 days no shedding on the 9th day，?whereas the goats only with EHEC O157:H7 were shedding bacteria for 23 days. Oral inoculation with O157:H7?(ΔhlyΔstxΔtoxB) was able to decrease shedding and colonization EHEC O157:H7 in goats. Therefore，our study?provides data to develop attenuated vaccine of EHEC O157:H7.

Key words: O157:H7(ΔhlyΔstxΔtoxB), Goats, Shedding bacteria

CLC Number:

ZHANG Xue-han, HE Kong-wang, YU Zheng-yu, MAO Ai-hua. Oral Inoculation with O157:H7(ΔhlyΔstxΔtoxB) Leads to Decreased Shedding in Goats after Challenge with Escherichia coli O157:H7[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 123-125. doi: 10.3969/j.issn.1000-7091.2012.03.024.

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Oral Inoculation with O157:H7(ΔhlyΔstxΔtoxB) Leads to Decreased Shedding in Goats after Challenge with Escherichia coli O157:H7

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