Identification of Biocontrol Bacteria against Soybean Root Rot with Biolog Automatic Microbiology Analysis System

doi:10.3969/j.issn.1000-7091.2012.02.045

Abstract

Abstract: In order to identify the systematic position of taxonomy of two biocontrol bacteria against soybean root?rot． Traditional morphological identification and BIOLOG automatic microbiology analysis system were used to identify strain B021a and B04b． The results showed that similarity value of strain B021a with Vibrio tubiashii was?0. 634，possibility to 86% and genetic distance to 4． 00，and similarity value of strain B04b with Pasteurella trehalosi?was 0． 610，probability to 75% and genetic distance to 2． 77． Strain B021a was identified as Vibrio tubiashii and?strain B04b as Pasteurella trehalosi by colony morphological propertie and BIOLOG analysis system．

Key words: Soybean, Root rot disease, Antagonistic bacteria, Biolog automatic microbiology analysis system, Identification

CLC Number:

S432

XU Yan-li, LIU Hai-long, LI Chun-jie, PAN Feng-juan, LI Shu-xian, LIU Xin-jing. Identification of Biocontrol Bacteria against Soybean Root Rot with Biolog Automatic Microbiology Analysis System[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 234-238. doi: 10.3969/j.issn.1000-7091.2012.02.045.

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References

[1] Baker R． Diversity in biological control[J]． Crop Protection，1991，10:85－94．
[2] 郭荣君，刘杏忠，杨怀文．大豆根际细菌拮抗大豆根腐?病研究[J]．大豆科学，1998，17(1):53－58．
[3] 郭荣君，刘杏忠，杨怀文，等．芽孢杆菌 BHl 防治大豆?根腐病的效果及机制[J]．中国生物防治，2003，19?(4):180－184．
[4] 程?亮，游春平，肖爱萍．拮抗细菌的研究进展[J]．江?西农业大学学报，2003，25(5):732－737．
[5] 刘海龙，李春杰，许艳丽．生防细菌的抑菌谱和对大豆根腐病的防治[J]．大豆通报，2008，92(1):10－13．
[6] 李春杰，刘海龙，许艳丽．大豆根腐病拮抗细菌的生物?学特性研究[J]．大豆科学，2011，30(5):809－812．
[7] Walther D． Biological control of damping off of sugar beet?and cotton with chaetomium globosum or a fluorescent?pseudomonas Sp1[J]． Canadian Journal of Microbiology, 1988，34:631－637．
[8] 余?建，张志元，高必达．细菌在植物病害生物防治中?的应用[J]．江西农业学报，2007，19(8):53－55．
[9] 杨秀荣，刘水芳，孙淑琴，等．生防细菌防治土传病害?的研究进展[J]．天津农业科技，2008，14(4):38－42．
[10] 段永照，刘?歆，潘竟军．拮抗生防细菌对土传性病?害的抑菌机理及应用现状[J]．新疆农业科技，2010?(3):60．
[11] 程?池，杨?梅，李金霞，等． Biolog 微生物自动分析?系统－细菌鉴定操作规程的研究[J]．食品与发酵工?业，2006，32(5):50－54．
[12] 潘?见，黄训端，谢慧明，等．草莓采后卫生微生物学?检查及细菌 VITEK 鉴定实验[J]．食品科学，2007，28?(3):320－323．
[13] Stager C E，Davis J R． Automated systems for identification of microorganisms[J]． Clinical Microbiology Reviews，1992，5:302－307．
[14] 焦?玲，程?英，田?刚．全自动微生物分析系统简?介及应用[J]．检验医学与临床，2010，7(18):2043－?2044．
[15] 刘?鹏，魏亚东，崔铁军． Biolog 系统和 16SrDNA 序列?分析方法在植物病原细菌鉴定中的应用[J]．植物检?疫，2006，20(2):86－87．
[16] Isenberg H D． Interp retation of aerobic bacterial growth?on primary culture media[M]/ /Isenberg H D． Clinical?microbiology procedures handbook，(Vol1)． Washington?DC:ASM，1992:1－9．
[17] 东秀珠，蔡妙英．常见细菌系统鉴定手册[M]．北京:?科学出版社，2001．
[18] 沈?萍，范秀容，李广武．微生物学实验[M]．第 3 版．?北京:高等教育出版社，2004．
[19] 王光华，金?剑，徐美娜，等．生防细菌 BRF-1 和 BRF2 鉴定及生物学特征[J]．中国生物防治，2007，23?(1):49－54．
[20] ?Hosoda A，Sakal M． Isolation and characterization of?agar-degrading Paenibacillus spp． associated with the?phizosphere of spinach． biosci． biotechno1[J]． Biochem，2003，67(5):1048－1055．
[21] Castro D，Pujalte M J，Lopez-Cortes L，et al． Vibrios Isolated from the cultured manila clam(Ruditapes philippinarum):numerical taxonomy and antibacterial activities
[J]． Journal of Applied Microbiology，2002，93(3):437?－443．
[22] Delston R B，Kothary M H，Shangraw K A，et al． Isolation and characterization of a zinc-containing metalloprotease expressed by Vibrio tubiashii[J]． Canadian Journal?of Microbiology，2003，49(8):525－529．
[23] Kunihiro Y，Keisuke G T，Tohru S． Identification and tissue expression analysis of C-type lectin and galectin in?the Pacific Oyster，Crassostrea Gigas[J]． Biochemistry?and Molecular Biology，2008，149(1):168－175．
[24] 杜宗军，王?鹏，李?筠，等．两株琼胶酶高产细菌的?筛选和鉴定[J]．海洋科学，2002，26(3):1－4．
[25] Heather J M，Patricia E S，Reggie Y C L． Novel protease?produced by a Pasteurella trehalosi serotype 10 isolate?from a pneumonic bighorn sheep:characteristics and potential relevance to protection[J]． Veterinary Microbiology，2003，93:145－152．
[26] Villard L，Gauthier D，Lacheretz A，et al． Serological and?molecular comparison of Mannheimiahae molytica and?Pasteurella trehalosi strains isolated from wild and domestic ruminants in the French Alps[J]． Veterinary Microbiology，2006，171:545－550．

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