Abstract:
In order to improve the quality and efficiency,reduce the cost of total RNA isolation from different plant tissues A method for effective isolation of total RNA from different plant tissues was invented based on hot borate method.The total RNA isolating from plant root,stem,leaf,fruits of different ripening stages,seeds of different ripening stages,pedicle of different ripening stages using this method had high purity and integrality detected by ultraviolet spectrophotometer and agarose gel electrophoresis.The A260/A230 of yielded total RNA was more than 2.3 while A260/A280 ranged from 1.8 to 2.0.The RNA can be applied to RT-PCR and Real time RT-PCR.This method can meet with demands of down stream experiment of common molecular biology and is an ideal method of plant total RNA extraction.
Key words:
Plant tissue,
Total RNA,
Hot Borate method
CLC Number:
REN Jie, XU Xiu-hong, ZHANG Su-li, LENG Ping. A Method for Effective Isolation of Total RNA from Different Plant Tissues[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 35-38. doi: 10.7668/hbnxb.2011.S1.007.