Application of Arbuscular Mycorrhizal Fungi on Vegetable Seedlings Culture with Different Substrates

doi:10.7668/hbnxb.2011.02.033

Abstract

Abstract: To investigate the possibility of applying arbuscular mycorrhizal fungi on vegetable seedlings culture, experiments were conducted with cucumber and lettuce as test crops to study the influence of arbuscular mycorrhizal fungi infection rate and growth.Seeds of cucumber and lettuce were sown in mixed substrate with various proportion of peat by volume(Peat：Perlite：Vermiculite=6：3：1；Peat：Vermiculite=1：4；Peat：Perlite=1：4；Peat：Polyfoam=I:4；Peat:Ceramsite=1：4)inoculated with AM fungi.AM fungi Glomus intraradics,Glomu mosseae,Glomus aggregeturn,Glomus etunicatum were used.The results showed the infection rate of AMF were above 24.17%in mixture of Peat+Ceramsite,while in Peat+Perlite+Vermiculite inoculated with Glomus intraradics was 34.05%,41.09%respectively,the others was below 20%.The seedling vigorous index are relatively high in media Peat+Ceramsite and Peat+Vermiculite,while in Peat+Perlite and Peat+Polyfoam both mycorrhizal and non-mycorrhizal treatment are low.It is thus clear that PeaCeramsite(1：4)treatments resulted in the most beneficial effects on seedling vigorous index and infection rate of crop roots,therefore Peat+Ceramsite was an ideal substrate for mycorrhizal seedling of vegetables.

Key words: Arbuscular mycorrhizal fungi, Infection rate, Grow seedlings, Substrates

CLC Number:

S182

WANG Hong-ju, WANG You-shan, ZHANG Shu-bin, ZUO Qiang, ZHANG Yong-qing, ZOU Guo-yuan. Application of Arbuscular Mycorrhizal Fungi on Vegetable Seedlings Culture with Different Substrates[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 152-156. doi: 10.7668/hbnxb.2011.02.033.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.02.033

http://www.hbnxb.net/EN/Y2011/V26/I2/152

References

[1] 吕桂云,陈贵林,齐国辉,等.蔬菜作物VA菌根研究进展[J].河北农业大学学报,2001(1):99-103.

[2] Chandanie W A,Kubota M,Hyakumachi M.Interactions between the arbuscular mycorrhizal fungus Glomus mosseae and plant growth-promoting fungi and their significance for enhancing plant growth and suppressing damping-off of cucumber(Cucumis sativus L.)[J].Applied Soil Ecology,2009,41 (3):336-341.

[3] Ghazala Nasim.The role of Arbuscualr mycorrhizae in inducing resistance to drought and salinity stress in crops[J].Plant Adaptation and Phytoremediation,2010 (1):119 -141.

[4] Zhang Li-dan,Zhang Jun-ling,Christie Peter.Pre-inoculation with arbuscular mycorrhizal fungi suppresses root knot nematode (Meloidogyne incognita) on cucumber(Cucumis sativus)[J].Biology and Fertility of Soils,2008,45 (2):205-211.

[5] 李小川.茄子工厂化育苗技术中基质穴盘肥料优化研究[J].山西农业科学,2008,36(7):52-54.

[6] 吕桂云,陈贵林,齐国辉.黄瓜菌根化育苗基质的研究[J].中国蔬菜,2002(4):9-12.

[7] 贺超兴,张志斌,王怀松,等.丛枝菌根真菌对番茄苗期生长及矿质营养吸收的作用[J].中国蔬菜,2006(1):9-l1.

[8] 王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2000:118-119.

[9] Horst V,Andrew P C,Urs W.Ink and vinegar,a simple staining technique for arbuscular-mycorrhizal fungi[J].Applied and Environmental Microbiology,1998,64 (12):5004-5007.

[10] Trouvelot A,Kough J L,Gianinazzi Pearson V.Mesure du taux de mycorhization VA d'un systeme radiculaire.Recherche de methods destimation ayant une signification fonctionnelle[M]∥Gianinazzi Pearson V,Gianinazzi S.Physiological and Genetical Aspects of Mycorrhizae.Paris:INRA Press,1986:217-221.

[11] 吕桂云.丛枝菌根化育苗对大棚黄瓜生长发育和果实品质的影响[D].保定:河北农业大学,2001.

[12] 孟凡枝,杨鹏鸣.不同施肥水平对三色堇根冠比和壮苗指数的影响[J].中国农学通报,2010,26(6):216-218.

[13] Sasai K.Infection of vesieular-arbuseular mycorrhizal fungi to plants and spore numbers in cultivated soils.In:Migayl Prefefcture[J].Seientific Reports of the Miyagl Agriculture College,1992,40:1-10.

[14] Masanori S,Takuya M.Inoculation with arbuscular mycorrhizal fungi:the status quo in Japan and the future prospects[J].Plant and Soil,2002,244:273-279.

[15] Dehne H W,Bachhaus G F.The use of Vesicular arbuscular mycorrhizal fungi in plant production I:lnoculum production[J].Journal of Plant Disease Protection,1986,93:415 -424.

[16] Feldmann F,Idczak E.Inoculation production of vesieular arbuscular mycorrhizal fungi for use in tropical Nurserise[M]∥Norris J R,Read D J,Varma A K.Techniques for Mycorrhizal Research.Lodon:Academic Press,1992:799-817.

[17] 闫杰,罗庆熙,韩丽萍.工厂化育苗基质研究进展[J].中国蔬菜,2006(2):34-37.

[18] 石兆勇,刘润进,李瑞卿.栽培基质与AM真菌对园艺作物的影响[J].中国生态农业学报,2002,10(2):50-52.

[19] 盖京苹,刘润进.土壤因子对野生植物AM真菌的影响[J].应用生态学报,2003,14(3):470-472.

[20] 秦海滨,贺超兴,张志斌,等.丛枝菌根真菌对温室有机土栽培黄瓜的作用研究[J].内蒙古农业大学学报,2007,28(3):69-72.

[21] 贺忠群,贺超兴,张志斌,等.不同基质接种丛枝菌根真菌对番茄生长及PAL、PPO酶活的影响[J].农业工程学报,2005,21(增刊):169-172.

[22] 任志雨,贺超兴,李树和,等.丛枝菌根直菌对有机栽培黄瓜生长、产量和品质的影响[J].华北农学报,2008,23(6):139-142.

[23] 张美庆,王幼珊,邢礼军,等.VA真菌耐高磷营养菌株筛选[J].华北农学报,1995,16(3):76-79.

[24] 陆文龙,郑鹤龄,潘洁,等.非灭菌条件下VA菌根真菌促进玉米吸收磷、氮营养的效应[J].华北农学报,1994,15(4):94-97.

Please choose a citation manager

Content to export