Abstract: In this study,the design was used to optimize AFLP-PCR amplification system on Aquilegia on four levels of four factors(Mg^{2 +}, dNTPs,Taq polymerse and primer). The amplification was performed using 64 pairs of primer combination and the products were separated on 6% polyacrylamide gels. 9 pairs of primer combination can produce scorable,clear,suitable and polymorphic bands. According to these experiments,the AFLP-PCR amplification system of Aquilegia was constucted and AFLP fingerprint was obtained. This optimized system for AFLP would become one of the protocols for further research on Aquilegia.

Key words: AFLP, Aquilegia, PCR, PAGE

CLC Number: 

• S68.03