Abstract: Embryogenic callus and regenerated plants were obtained from hypocotyl explants of glandless upland cotton seedling. The results indicated that hormone was an important factor that effected cotton direct somatic embryogenesis. 2, 4-D was advantageous to the induction of cotton callus, but embryogenic callus could not be directly obtained on the MSB sold medium with 2, 4-D. Embryogenic callus were not directly obtained on the MSB sold medium with the combination of IBA and BA. Embryogenic callus could be directly obtained on the MSB sold medium with the combination of IBA and KT. The MSB sold medium with the concentration of 1.0 mg/L IBA and 0. 5 mg/L KT could induce a number of embryogenic callus from hypocotyl explants. Somatic embryos could be observed within three months. The MSB sold medium with the concentration of 1.0 g/L Gin and 0.5 g/L Ash could promote the germination and maturation of the somatic embryos. In this procedure, a number of regenerated plants could be obtained in six months. In the present study, a simple and efficient system was developed to induce a number of embryogenic callus and regenerated plants from hypocotyl explants.

Key words: Cotton, Glandless, Somatic embryogenesis, Plant regeneration

CLC Number: 

• S562.03