Molecular Identification of A Purple Chinese Cabbage CMS Material

doi:10.7668/hbnxb.2009.03.037

Abstract

Abstract: In order to identify and certify the genotype of a purple Chinese cabbage CMS, and use the purple character - istics in Chinese cabbage, orF₁38 primerswere used for PCR analysis in 6materials including purple Chinese cabbage CMS, maintainer line 07 -721, F₁, 3 back -crossing segregating plants BC₁ -1, BC₁ -2, BC₁ -3.Then the PCR products were cloned, se - quenced and aligned.Results showed 309bp fragments could only be amplified in purple Chinese cabbage CMS, F₁, BC₁ -1, BC₁ -2, BC₁-3, excluding maintainer line 07 -721. Alignment results showed orF₁38 in purple Chinese cabbage CMS was ho - mologous to Ogu CMS in Raphanus sativus and cybrids of Brassica crops with Ra phanus sativus.The identities among them were 99%. E value was 2e -155. Therefore, the purple Chinese cabbage CMS material belongs to an improved Ogu CMS genotype.The application of the CMS genotype and purple genes in the future is discussed.

Key words: Purple Chinese cabbage, Ogu CMS, orF₁38, Application

CLC Number:

S634.1

ZHANG De shuang, CHEN Bin, ZHANG Feng lan, YU Yang jun, ZHAo Xiu yun, YU Shuan cang, XU Jia bing. Molecular Identification of A Purple Chinese Cabbage CMS Material[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 174-178. doi: 10.7668/hbnxb.2009.03.037.

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