Cloning and Expressing of the Fragment of Porcine Circovirus2 ORF2 Gene

doi:10.7668/hbnxb.2008.04.012

Abstract

Abstract: The behind fragment of PCV2 ORF2 gene was amplified from the extracted DNAof PCV2 in inoculated in PK-15 cells;The PCR product was connected with pMD19-T vector,which was confirmed by sequence analysis and re-strict enzyme identification,and then was cloned into pET- 32aVector,Plasmid containing the right insertion was digested with enzymes to confirm its identity and retransformed the recombinant plasmid into E. coli BL21 strain. The bacteria were induced by IPTGand the lysates were analysed directly by SDS-PAGE.Results were asfollows. Afusion protain ap-proximately 40 kDa in molecular weight was observed on the SDS-PAGE. Western-blotting confirmed that had antigenic activity of PCV2.

Key words: PCV2, ORF2 gene fragment, Clone, Prokaryotic expression

CLC Number:

S432.4+1

ZHANG Xiu-shan, CAO Hong-zhan, ZHANG Li-qing, LIU Tao, CHEN Sai-juan, SUN Ji-guo. Cloning and Expressing of the Fragment of Porcine Circovirus2 ORF2 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 51-54. doi: 10.7668/hbnxb.2008.04.012.

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