Abstract: To study the optimal technical conditions of shaking fermentation for producing β-mannanase by Bacillus circulans WXY-100,the culture ingredients and conditions were optimized with the orthonormal table L₉（3⁴） method.Studies showed that the culture components for optimization of the enzyme production were yeast extract 20g/L,Konjak powder 20g/L,（NH₄）₂SO₄ 2g/L,KH₂PO₄ 1g/L and MgSO₄ܭH₂O 1g/L;and the optimal culture conditions were seed incubation time 8~12 h,inoculation ratio 6%,medium volume 25 mL,pH 7.5,fermentation temperature 40℃ and fermentation time 25 h.The enzyme was stable between pH 4-8 and below 60℃.The optimal pH and temperature for enzyme activity were 5.0 and 60℃ respectively.Cu²⁺ and Co²⁺ had a remarkable influence on increasing the activity of β-mannanase,while Hg²⁺ was on the contrary.

Key words: B-mannanase, Bacillus circulans, Optimal conditions, Properties

CLC Number: 

• S556