Identification of Heterorhabditis spp.Populations Isolated from Hebei Province

doi:10.3321/j.issn:1000-7091.2005.05.022

Abstract

Abstract: 40 Heterorhabditis spp.populations isolated from Hebei province were identified based on the morphological and molecular characters.The main morphometrical characteristics based on the average of four typical populations are as followed:infective juvenile length=550(520-610)μm,tail length=85.7(80-88)μm,body width =22.2(20-24)μm,E%=112.5%(105%-119%);Male spicule =36.8(33.7539.0)μm,gubernaculum length =17.9(16.3-20.0)μm,GS%=0.49(0.41-0.59),SW=1.25(1.15-1.43),they are mostly close to H.bacteriophora in morphology.The ITS-rDNA of two local populations was sequenced.The ITS1-rDNA sequence differences are less than 1.2% between local populations and H.bacteriophora,less than 1.5% between local populations and H.argentinensis,but over 18.7% between local populations and other known species.It is proved that the Heterorhabditis spp.populations isolated from Hebei province are H.bacteriophora based on morphological and molecular characters.

Key words: Entomopathogenic nematodes, Heterorhabditis bacteriophora, Ident ification, Biological control

CLC Number:

S435.622

CHEN Shu-long, LI Xiu-hua, Maurice Moens. Identification of Heterorhabditis spp.Populations Isolated from Hebei Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(5): 89-95. doi: 10.3321/j.issn:1000-7091.2005.05.022.

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