Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2001, Vol. 16 ›› Issue (4): 77-80. doi: 10.3321/j.issn:1000-7091.2001.04.016

Special Issue: Corn； Biotechnology；

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Optimization of Parameters Influencing Gene Delivery into Maize Calli by Gene Delivery System

JIA Weilong1, YANG Lili1, ZHOU Xiaomei2, WANG Jingxue2, SUN Yi3   

  1. 1. Arid Farming Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan Shanxi 030031, China;
    2. Department of Life Science, Shanxi University, Taiyuan Shanxi 030006, china;
    3. Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan Shanxi 030031, China
  • Received:2000-07-05 Published:2001-12-28

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Abstract: Using maize calli as recipient cell,transforming parameters of PDS 1000/He gene delivery system and self made gene gun have been studied through GUS transient expression.The most suitable delivery parameters for PDS 1000/He gene delivery system are 1350psi of pressure,25 In·Hg of vacuum degree and 6 cm shoot distance of microcarrier;and for self made gene gun are 0.6 MPa pressure,10 c shoot distance of microcarrier.

Key words: Maize calli, Gene gun, GUS transient ex pression rate, GUS transient expression unit

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Cite this article

JIA Weilong, YANG Lili, ZHOU Xiaomei, WANG Jingxue, SUN Yi. Optimization of Parameters Influencing Gene Delivery into Maize Calli by Gene Delivery System[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(4): 77-80. doi: 10.3321/j.issn:1000-7091.2001.04.016.

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