Comparative Analysis of Polymorphism of SSRs Detected on Two Gel Electrophoresis Systems

doi:10.3321/j.issn:1000-7091.2001.02.009

Abstract

Abstract: Thirty five SSR primers and 14 maize inbred lines were used to comparatively analyze polymorphic level of SSRs on two gel electrophoresis systems.The results showed that the number of alleles and PIC value over 35 SSR primers were significantly different between two gel systems.Polyacrylamide gel(12%) had higher ability than Metaphor agarose gel(3%) in discriminating amplified fragments.On the other hand,the correlation coefficient of genetic similarities between 14 lines obtained on two gel systems was 0 516,significantly correlated at P0 01 level,indicating that the information explored by two gel systems were relatively similar.Eight SSR primers of 35 primers were identified to have high PIC value and number of alleles over 14 lines on both two gel systems,and could be used for unique DNA fingerprinting of each inbred line tested.Based on the results,3% Metaphor agarose gel was suggested to be used in genetic mapping,while 12% polyacrylamide gel would be used in DNA fingerprinting or genetic variation studies.

Key words: Maize, SSR marker, Genetic variation, Polymorphic information content(PIC)

CLC Number:

S513.03

Li Haixin, Jiao Shaojie, Fu Junhua, Zhang Shihuang, Yuan Lixing. Comparative Analysis of Polymorphism of SSRs Detected on Two Gel Electrophoresis Systems[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(2): 43-48. doi: 10.3321/j.issn:1000-7091.2001.02.009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2001.02.009

http://www.hbnxb.net/EN/Y2001/V16/I2/43

[1]	ZHAI Lichao, ZHANG Lihua, ZHENG Mengjing, LÜ Lihua, SHEN Haiping, YAO Haipo, JIA Xiuling. Responses of Kernel Position Effect of Summer Maize to Increasing Plant Density and Its Carbon and Nitrogen Metabolism Characteristic [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 97-104.
[2]	LIU Shuai, XU Xuexin, ZHAO Jinke, QU Wenkai, HAO Tianjia, MENG Fangang, JIA Jing, ZHAO Changxing. Effects of Nitrogen Application Rate and Frequency on Photosynthetic Characteristics,Protective Enzyme Activities and Yield of Summer Maize under Drip Irrigation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 114-123.
[3]	FU Jingfeng, LUO Shangke, CHENG Yi, ZHANG Jun, WEI Sheng, ZENG Tao, CHEN Du, WEI Pengcheng, ZHENG Yingxia, SONG Bi. Effects of Different Film Mulching Methods and Nitrogen Application Rates on Yield and Nitrogen Absorption and Utilization of Spring Maize [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 158-168.
[4]	LI Qiang, KONG Fanlei, YUAN Jichao. Cultivar Differences in Nitrogen Uptake,Utilization and Field Nitrogen Balance of Maize Hybrids in Response to Nitrogen Management [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 169-181.
[5]	ZHAO Siqi, QUBI Wuhe, LUO Ting, YU Xuejie, KE Yongpei, GOU Qixian, SHI Haichun. Physiological and Biochemical Responses of Maize to Gray Leaf Spot Stress [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 190-197.
[6]	LING Chen, ZHAO Hong, YAN Jun, MA Yingxue, FENG Yanfang, ZHANG Jingli, LI Bo, DENG Chao, XU Zhenjiang. Establishment and Application of Variety Identification System Based on SSR Markers for Lettuce [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 34-44.
[7]	WANG Yongchao, YAN Bowen, CAO Hongzhang, WANG Shancong, MA Mengjin, ZHANG Junjie, GUO Jiameng, WANG Hao, SHAO Ruixin, YANG Qinghua. Effects of the Mixture of DA-6 and CCC on the Photosynthesis and Anti-Premature Senescence of Maize Leaves under Dense Planting [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 90-102.
[8]	ZHAO Changjiang, DU Mengxiang, SONG Juqi, XU Shangyuan, HE Lin, XU Jingyu, YANG Kejun, LI Zuotong. Genome-wide Identification and Expression Response to Stresses Analysis of NRL Gene Family in Zea mays [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 1-10.
[9]	ZHANG Zongxiang, HUANG Zhengrong, WU Xuefan, LIU Nannan, LI Xiaoxiao, DONG Zhaorong, SONG He. Effects of Soil Acidification on Yield,Nitrogen Metabolism, and Related Gene Expression of Maize [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 94-103.
[10]	TAI Jicheng, HAN Meiqi, YANG Hengshan, ZHANG Yuqin, MA Jinhui, GUO Xiaoxu. Effects of Urea Reduction with UAN on Dry Matter Accumulation and Nitrogen Efficiency of Spring Maize under Shallow Buried Drip Irrigation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 136-144.
[11]	LIU Jianling, WU Jing, JIA Ke, LIAO Wenhua, LÜ Yinghua, MA Junyong. Trends of Yield Responses to Soil Phosphorus and Phosphate Fertilizer in Winter Wheat-Summer Maize Rotation System [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 104-111.
[12]	CAI Fengle, MA Xin, WANG Shuaili, LU Liangtao, SHAO Ruixin, LI Hongping, ZHAO Yali, MU Xinyuan, ZHAO Xia, LI Shuyan, LIU Tianxue. Effect Regulating of Nitrogen on Grain Development of Maize under Heat Stress [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 119-127.
[13]	ZHANG Panpan, SHAO Yunhui, LIU Jingbao, QIAO Jiangfang, LI Chuan, ZHANG Meiwei, ZHAO Xia, HUANG Lu. Effect of Nitrogen and Zinc Application on the Accumulation and Distribution of Dry Matter and Nitrogen and Zinc of Different Summer Maize Varieties [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 96-103.
[14]	SU Gang, WANG Yizhen, GE Junzhu, LI Zifang, ZANG Fengyan, WANG Jinlong, WU Xidong. Effects of Green Manure Rape Returning Stages and Different Nitrogen Application Rates on Yield of Spring Maize and Soil Nutrients [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 121-131.
[15]	JIA Liqiang, LIU Xun, DING Bo. *The Expression Pattern of ZmbZIP* Gene under Adversity Stresses** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 9-17.

Please choose a citation manager

Content to export