Abstract: Using ND La Sota strain,respiratory tract type IB(RIB)H₁₂₀ strain or kidney type IB(KIB)SN₉₃₀₁ strain and EDS XN₉₂₀₃ strain as vaccine strains.After proliferation through chicken and duck embryos,inactivate with formaldehyde.Mix the inactivated viral liquid with oil adjuvant as per the viral titer to produce by means of emulsification "ND-RIB-EDS" and "ND-KIB-EDS" triplex oil emulsion vaccine.the prepared triplex vaccine shows that:1：The vaccine is safe itself ,having no side effects to chicken,being good in the degree of emulsification,stableness and stylet.2:The immunologic effect is good.Immunize chickens of 20 days with the triplex vaccine.On the 14th,28th and 58th day after immunization,the antibodies of ND,RIB,KIB and EDS can more than 7.0 and 9.2 log2.60 days after immunization,challenge with the virulent strain of ND,RIB and KIB,83.3% to 100% of the immunized chickens are effectively protected.3:The Preservation period is long,being 180 days.(room temperature and out of reach of light )and 360 days (about 4℃).The immunologic effect shows no obvious difference with newly-prepared vaccine.

Key words: Newcastle disease, Infectious bronchitis, Egg drop syndrome, Triplex oil emulsion vaccine

CLC Number: 

• S859.79