Functional Analysis of PmSOC1 -like Genes Related to Floral Organ Development in Prunus mume

doi:10.7668/hbnxb.20190196

Abstract

Abstract: The MADS-box gene SOC1/TM3 (SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1/Tomato MADS-box gene 3) integrates multiple flowering signals to regulate the transition from vegetative to reproductive development in plants. Three putative orthologs of SOC1 (PmSOC1-1, PmSOC1-2 and PmSOC1-3) were cloned from Prunus mume Changruilüe. The sense plant expression vectors driven by Cauliflower mosaic virus 35S promoter were constructed,and then were transformed separately into wild-type Arabidopsis plants, to study the function of PmSOC1-1, PmSOC1-2 and PmSOC1-3. Overexpression of PmSOC1-1, PmSOC1-2, and PmSOC1-3 in Arabidopsis caused early flowering. Early flowering also increased expression levels of four other flowering promoters, agamous-like 24 (AGL24), leafy (LFY), apetala 1 (AP1) and fruitfull (FUL). Moreover, the overexpression of PmSOC1-1 and PmSOC1-2 resulted in a range of floral phenotype changes such as sepals into leaf-like structures, petal color into green, petal into filament-like structures. These results suggested that the genes PmSOC1-1, PmSOC1-2 and PmSOC1-3 played an evolutionarily conserved role in promoting flowering in P. mume, and might have distinct roles during flowering development. These findings will help elucidate the molecular mechanisms involved in the transition from vegetative to reproductive development in P. mume.

Key words: Prunus mume, PmSOC1 -like, Early flowering, Functional analysis

CLC Number:

S685.17

LI Yushu, YANG Weiru, YANG Yujie, CHENG Tangren, WANG Jia, ZHANG Qixiang. Functional Analysis of PmSOC1 -like Genes Related to Floral Organ Development in Prunus mume[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 74-82. doi: 10.7668/hbnxb.20190196.

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