Abstract: In order to study the influence of different application amounts of charcoal on the microbial diversity in tobacco rhizosphere soil, with the rhizosphere soil of flue-cured tobacco variety Yunyan 87 as the research object, we compared the situation by applying different amounts of charcoal, and conducted high-throughput sequencing of rhizosphere soil ITS2 region and 16S rDNA-V4 region with progressive treatment with 3 application amounts of charcoal; through bioinformatics analysis of data, we obtained the OTU abundance, distribution,α diversity, community species composition and abundance information of microorganisms in rhizosphere soil when different treatment methods were used, and conducted PCA clustering analysis and UPGMA clustering analysis of the community composition and abundance. The results showed that within the application scope of 50-150 g/tree, by increasing the application amounts of charcoal, it could increase the variety diversity and distribution uniformity of bacteria in rhizosphere soil, while it would reduce the variety diversity and distribution uniformity of fungus; compared to the control group where no charcoal was applied, after applying the charcoal with the amounts of 50, 100,150 g/tree, the abundances of Proteobacteria bacteria had declined by 4.1%, 2.7% and 0.7% respectively; the abundances of Acidobacteria bacteria increased by 10.4%, 8.1% and 7.7% respectively; the abundances of Actinobacteria bacteria were all lower than the abundance of control group; the difference in abundance of Gemmatimonadetes bacteria between various groups was very small. After applying the charcoal with the amount of 50 g/tree, the abundance of Zygomycota fungus had declined by 12.38% compared to the control group(50.92%), and then, with the increased of the application amount of charcoal, the abundance of Zygomycota fungus gradually increased to 53.68%; on the contrary, after applying the charcoal with the amount of 50 g/tree, the abundance of Ascomycota fungus had increased by 10.15% compared to the control group(30.63%), and then, with the increased of the application amount of charcoal, the abundance of Ascomycota fungus gradually declined to 29.11%; after applying the charcoal with the amount of 50 g/tree, the abundances of Basidiomycota and Chytridiomycota funguses almost had no change, and then, with the increased of the application amount of charcoal, the abundances of Basidiomycota and Chytridiomycota funguses presented the trend of increased first, and then declined; various treatment methods did not have significant influence on the abundance of Glomeromycota fungus. By studying the influencing method and action rules of charcoal application on the micro-ecology of tobacco rhizosphere, it can provide theoretical basis to the application of charcoal in tobacco field.

Key words: Biochars, Tobacco, Rhizosphere soil, Microbial, Diversity

CLC Number: 

• Q939.96