Abstract: Cloning a male sterility gene from foxtail millet male sterility material 1066A, analyzing the mutation sites between sterility gene and fertile gene, which could provide foundation for exploring molecular mechanism of male sterility, breeding multi-purpose male sterility materials by marker assisted selection method. A putative male sterility gene was cloned from 1066A based on the complete foxtail millet genome sequence data and location results of predecessors to explore mutation sites leading to sterility,which could provide foundation for uncovering male sterility mechanism at molecular level and breeding multi-purpose male sterility materials by marker assisted selection method.Firstly,a male sterility gene with total length of 5 027 bp (coded 479 aa,Si015780m.g) was found on chromosome 6 of foxtail millet cultivar Yugu 1 by bioinformatics method,and the gene was in the same genome region as that located by molecular markers. Two pairs of specific primers were designed according to male sterility gene sequence of Yugu 1 to amplify corresponding male sterility gene of 1066A (a male sterility material).Totally 2 561 bp gene sequence was obtained after assembling of two amplification fragments,which contained part code region downstream of the gene.After sequence alignment of part coding regions and putative protein sequences of male sterility gene from Yugu 1,Zhanggu and 1066A,three mutation sites including two single base substitutions and one base insertion were found in coding region of male sterility gene from 1066A.Two base substitutions led to Ile (402) and Leu (403) from protein of Yugu 1 and Zhanggu were replaced by Val and Ile from protein of 1066A,and one base insertion led to premature translation termination of protein from 1066A when reached the 466th aa.Of the three mutation sites, two aa substitutions had limited effect on function of coding protein,so we suggested that premature translation termination of protein from 1066A male sterility gene would be the reason for its sterility phenotype.This study provided foundation for uncovering molecular mechanism of male sterility in foxtail millet.

Key words: Foxtail millet, 1066A, Male sterility, Clone, Sequence alignment

CLC Number: 

• S565.03

• Q78