Cloning and Bioinformatics Analysis of Elastinolytic Metalloproteinase Gene from Puccinia helianthi Schw

doi:10.7668/hbnxb.201751261

Abstract

Abstract: In order to study the pathogenic mechanism of Puccinia helianthi Schw, bioinformatics method was used analyze the mature part of KU994904 gene that had a high expression level in the transcriptome of Puccinia helianthi Schw. The protein ORF region was analyzed by Interpro which can determine its mature region. Using the bioinformatical databases and softwares, its function was analyzed and predicted. Total RNA was extracted from Puccinia helianthi Schw and used to amplify the mature gene of the metalloproteinase by RT-PCR. Finally, the gene sequence of mature protein was cloned. The results showed that the sequence full-length of the gene was 1 335 bp, coding 445 amino acids. And the protein molecular weight was 49.5 ku, it was found that the gene had high similarity with five sequences of M36 family and the gene had 53.77% similarity with the extracellular metalloproteinase (mep). So it was deduced that the gene coded an elastinolytic metalloproteinase.

Key words: Puccinia helianthi Schw, Elastinolytic metalloproteinase, Cloning, Bioinformatics analysis

CLC Number:

Q78
S432.01

LI Xinchun, SONG Yang, LU Yan, JING Lan. Cloning and Bioinformatics Analysis of Elastinolytic Metalloproteinase Gene from Puccinia helianthi Schw[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 186-193. doi: 10.7668/hbnxb.201751261.

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