Cloning,Construction of Expression Vector and Expression Analysis of NtPP2C16 in Nicotiana tabacum

doi:10.7668/hbnxb.2017.05.012

Abstract

Abstract: 2C protein phosphatase (PP2C) is a key component in the ABA signal transduction pathway and plays a pivotal role in plant growth and development,cell cycle regulation and adaptation to adversity stresses.To explore the function of the PP2C gene in tobacco adaptation to abiotic stress,a PP2C homologous gene was cloned from the tobacco cultivar K326,which contained a 1 617 bp ORF encoding 538 amino acid. The homology analysis showed that the gene had high homology with PP2C16 of Nicotiana tomentosiformis. So it was named NtPP2C16. Bioinformatics analysis showed that the NtPP2C16 catalytic region had 11 structural sub-regions which were relatively conservative in the PP2C family evolution. qRT-PCR analysis revealed that the expression of this gene was significantly induced by ABA and H₂O₂ signal molecules,and responded to drought,high salt,low temperature and low potassium stresses. NtPP2C16-pBI121 overexpression vector was constructed successfully,the results provided some basis for analysis of NtPP2C16 responsing to abiotic stresses in tobacco.

Key words: Tobacco, NtPP2C16, Cloning, Sequence analysis, Expression

CLC Number:

Q78
S572.03

ZHANG Xuewei, LIU Lun, LU Liming, LI Liqin. Cloning,Construction of Expression Vector and Expression Analysis of NtPP2C16 in Nicotiana tabacum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 78-85. doi: 10.7668/hbnxb.2017.05.012.

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