Cloning and Expression Analysis of TIP4;1 Gene from Brassica napus

doi:10.7668/hbnxb.2016.02.007

Abstract

Abstract: Aquaporins are channel proteins, which located in biological membranes including plasma membrane and tonoplast.It can highly facilitate water transportation across biological membranes.To investigate molecular mechanism of water regulation during seed germination, a tonoplast intrinsic proteins(TIPs)gene fragment TIP4;1 was isolated from Brassica napus seed by RT-PCR.The expression ofTIP4;1 gene during seed germination and stresses treatment was analyzed by quantitative Real-time PCR(qRT-PCR).The transcription levels of TIP4;1 was analyzed during germination, and these results showed TIP4;1 expression levels was scarcely detected in dry seed, but was up-regulated during germination as well as early young seedlings.In addition, expression of TIP4;1 in response to abiotic stress was investigated, and results showed that TIP4;1 gene expression was upregulated under drought, cold and salt stress, suggesting that TIP4;1 may be involved in Brassica napus seed germination and stress response process.Aquaporin regulation mechanism would be further revealed in future, which had realistic significance for solving drought and salt stress problems.

Key words: Brassica napus, AQPs, TIPs, Seed germination, qRT-PCR

CLC Number:

GE Fengwei, JIANG Yi, ZHAO Huixin. Cloning and Expression Analysis of TIP4;1 Gene from Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 32-37. doi: 10.7668/hbnxb.2016.02.007.

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