Cloning and Sequence Analysis of a Novel Promoter of Polygalacturonase Gene in Pruns persica (L.)

doi:10.7668/hbnxb.2014.06.010

Abstract

Abstract: The objective of the study was to obtain the 5' flanking sequence of polygalacturonase gene from the genomic DNA of Pruns persica (L.)Batsch.Chromosome walking techniques and the software of PROMOTER PREDICTION and PLANT CARE online were used.The results showed that a novel promoter of PG gene was obtained,the length of it was 986 bp,alignment indicated that the sequence had 96% homology with that of the polygalacturonase gene.The core promoter regions and some upstream regulatory elements in this fragment were analyzed.Transcriptional start site(TSS)was C.The sequence of PG gene promoter contained several specific acting elements,They were TATA-box,CAAT-box,light-responsive elements,drought-induced MYB binding sites,salicylic acid response elements,and other cis-elements.The accession number was FJ940722.The studying suggested that the expression of the novel promoter of PG gene could be regulated by hormone,drought,light and other factors.

Key words: Pruns persica, Polygalacturonase (PG), Promoter, Sequence analysis

CLC Number:

S667.9
Q78

CHEN Di-xin, LI Yan-mei, GUO Guo-ning, CHI Hui, YANG Rui-juan, YANG Ying-jun. Cloning and Sequence Analysis of a Novel Promoter of Polygalacturonase Gene in Pruns persica (L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 52-57. doi: 10.7668/hbnxb.2014.06.010.

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