Research on Biological Effects and Molecular Markers of Maize Lines Implanted with Low Energy N~+ Ion Beam

doi:10.7668/hbnxb.2011.05.027

Abstract

Abstract: Three groups of dry seeds of maize inbred lines,including Zheng58,Chang7-2 and K12 were implanted with different doses of low energy N+ ion beam.We analyzed the dates of germination percentage,germinating energy and agronomic characters,and find that to the energy 30 kV,7×1016 N+/cm² was the best implanted dose.At the mean time,the cluster analysis was done by molecular markers with 10 pairs of SSR primers.39 alleles were detected,2-5 alleles per primer,average 3.9.Zheng58 of the three inbred lines implanted with N+ ion beam was the most sensitive.According to the cluster analysis,the best implanted dose was 7×1016 N+/cm² to the energy 30 kV.The results of the two methods were almost consistent.

Key words: N+ ion beam, Maize, Biological effects, Molecular markers

CLC Number:

S513

WANG Chuang-yun, WANG Lu-jun, QIN Zuo-xia, WANG Yan-mei, HOU Ya-jing, ZHANG Li-na, LI Hai-yan, WANG Mei-xia. Research on Biological Effects and Molecular Markers of Maize Lines Implanted with Low Energy N~+ Ion Beam[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 131-134. doi: 10.7668/hbnxb.2011.05.027.

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