Rapid Identification of Bactrocera dorsalis with Nested PCR

doi:10.7668/hbnxb.2010.01.046

Abstract

Abstract: Bactrocera dorsalis(Hendel),as oriental fruit fly,is a serious quarantine pest of fruits in tropical and subtropical countries.Its rDNA gene includes 18S RNA,ITS-1,5.8S RNA,ITS-2,and 28S RNA.In this study,a nested PCR was carried out for amplification of ITS fragments containing 5.8S RNA.The sequence of the PCR fragment was greatly similar with Bactrocera dorsalis(Hendel)′s in NCBI Genbank.The PCR technique can be used to rapidly identify eggs of Bactrocera dorsalis(Hendel) in fruit.

Key words: PCR, rDNA, ITS, Bactrocera dorsalis(Hendel), Molecular diagnostic

CLC Number:

S436.66

KONG Qiu-lian1,YE Jun3,YUE Ling1,CHEN Zhi-jun1,BAO Ying-zi2,XU Yun2,GUO Ka2,YUAN Zhong-yi2,QI Wen-yuan1. Rapid Identification of Bactrocera dorsalis with Nested PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 226-230. doi: 10.7668/hbnxb.2010.01.046.

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