Establishment of cDNA-AFLP Analysis System for Vitis amurensis Rupr and Screening Primers

doi:10.7668/hbnxb.2009.02.017

Abstract

Abstract: The cDNA-AFLP systemfor Vitis amurensis Rupr was established after optimizing several key factors affecting cDNA-AFLP analysis.The result indicated that CTAB method could be used for extracting ideal RNA from leaf and inflorescence of Vitis amurensis Rupr,while SDS-acidic phenol method for extracting RNA from bud;500 ng cDNA was digested completely by EcoR I and Mse I for 6 hours.The reducible results was obtained when the ligation products were diluted to 5 times for preamplification template and preamplification productsthe were diluted to 20 times for selective amplification.

Key words: Vitis amurensis Rupr, cDNA - AFLP

CLC Number:

S663.1

TANG Mei-ling, KONGJin, XU Wei-hua, HAN Zhen-hai. Establishment of cDNA-AFLP Analysis System for Vitis amurensis Rupr and Screening Primers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 79-83. doi: 10.7668/hbnxb.2009.02.017.

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