A Rapid RT-PCR Detection for the Three Kinds of Viruses in Tobacco

doi:10.7668/hbnxb.2007.S1.006

Abstract

Abstract: In order to extract the total RNA from the plant leaf and to detect the viruses of tabacoo rapidly, a extractionmethod and detection method were studied.The TRIzol Reagent box was used to extract the total RNA from the leavescucumber and N . tabacum cv. White burly .The total RNA with the high purity and integrality was obtained in 1 h. Based onthe genome conservative sequence of tabacoo mosaic virus, cucumber mosaic virus and potato X virus, special primers weredesigned respect ively.With the RT-PCR, the target fragments about 313, 326 and 392 bp were ampilfied separately from theinfected sample, but not health sample.There recombinant plasmids were constructed and their sequences were comparedseverally with these three kinds of virus nucleotide sequences in GeneBank. The homology achieve separately 99.36%,97.55% , 98.21%.These methods were rapid and reliable. It supplied scient ific base for the detection to viruses of tabacoo.

Key words: Tabacoo mosaic virus, Cucumber mosaic virus, Potato X virus, RT-PCR

CLC Number:

S435.72

GURui-hua, WANGJin-zhong, WENSi-yuan, WANGSheng-qi, ZHANGAi-huan, WEIYan-min. A Rapid RT-PCR Detection for the Three Kinds of Viruses in Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.006.

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