Abstract: The DnaJ-like proteins and related genes are frequentily studied which are related with heatshock and other stresses in plant recentily. Using RT-PCR we cloned a foxtail millet Dna-J like gene in this report. RNA isolation was carried out by the Trizol method with 0.8%NaCl solution treated foxtail millet seedling; first chain cDNA was obtained with the reverse transcripte enzyme M-MLV and then used as templated to PCR cloning the cDNA sequence of the foxtail millet Dna-J like genes. A PCR fragment of predicted size of 1.2 kb was cloned and sequenced. The cloned fragment contains 1260 bp sequence which encodes a protein of 419 amino acids. Both the DNA sequence and amino acid sequence show great similarity with their corresponding sequences from rice, maize and wheat. The predicted protein has the three conserved domains which characteristic the structure of Dna-J protein family members. The cloned foxtail millet Dna-J gene was constructed into an expression vector for furthwer functional and transformation study.

Key words: Foxtail millet, Dna-J, Gene cloning

CLC Number: 

• S515.03