Genotypic Identification of the S-c Locus in the Indica-compatible Japonica Lines of Rice (Oryza sativa L.)

doi:10.7668/hbnxb.2007.04.002

Abstract

Abstract: S-c is one of the F1 pollen sterility loci between indica and japonica cultivars in rice. The genotype of Taichong 65 (T65) and Guangluai 4 (GLA 4) at the locus is S^j/S^j and Sⁱ/Sⁱ, respectively. Four indica-compatible japonica lines (ICJLs), G24163, G2417-2-1, G2605 and G3004-4 were crossed with testers, T65 and GLA 4, and their S-c locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-c locus and plant pollen fertility in several F2 populations showed that G2416-3 possessed S^i-2/S^i-2 genotype; G2605 and G3004-4 possessed S^i-1/S^i-1 genotype; while G2417-2-1 possessed Sⁿ/Sⁿ genotype. The test methods on genetic differentiation of the F1 pollen sterility loci were also discussed in this paper.

Key words: Rice (Oryza sativa L.), Indica-compatible japonica lines, Pollen sterility, Multi-alleles, Microsatellite marker

CLC Number:

S511.03

DING Xiao-hua, ZHANG Ze-min, ZENG Rui-zhen, ZHANG Gui-quan. Genotypic Identification of the S-c Locus in the Indica-compatible Japonica Lines of Rice (Oryza sativa L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 5-8. doi: 10.7668/hbnxb.2007.04.002.

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