Abstract: Effects of nickel on the ultrastructural changes were studied in the broad bean and winter wheat seedling cells. Accompanying nickel uptake.the damage of root-tip cells was examined in both broad bean and winter wheat seedling in varying degrees. Change of nucleus shape, separation of cell membrane and wall were observed in the root-tip cells of broad bean exposed to 15μg/ml NiSO₄ for 4 days. A condensed substance appeared nn the inner membrane of vacuole intermittently or insi.le vacuole. Under the condition of same treaty ent concentration, no significant changes were observed in the root-tip cells of winter wheat seelling. After treatment with 80μg/ml NiSO₄ for 4 days, the vacuole showed irregular shape. A condensed substance inside vacuole, separation of cell membrane and wall were obsrerved in thr root-tip cells of winter wheat seedling. But at this concentration, broad bean was found to be more sensitive to nickelinduced damage than winter wheat seedling. Disintegration of nucleus membrane, condensation of nucleus, severe separation of cell membrane and wall, and increase of electron density for the cytoplasm were observed within root-tip cells of broad bean.

Key words: Nickel, Broad bean, Winter wheat, Cytotoxicity, Ultrastructure

CLC Number: 

• Q942