Cloning and Sequencing of－ gliadin Genes from Common Wheat Cultivar Zhengfeng 5

doi:10.3969/j.issn.1000-7091.2013.04.009

Abstract

Abstract: The－ gliadins are the important components of seed strorage proteins，and their composition and content play a major role in determining the processing quality of wheat．Using PCR technology，total 32 novel genes (Designated as ZF5A- 1－ ZF5A-32; GenBank No: JX828280－JX828311 ) were cloned from the wheat cultivar Zhengfeng 5，including 17 full- ORF－ gliadin genes(Designated as ZF5A-1－ZF5A-17) and 15 pseudogenes.The sequence analysis of deduced amino acids showed that 10 had the typical structural characteristics of－ gliadin genes reported previously，and 7(ZF5A-1，ZF5A-3，ZF5A-6，ZF5A-9，ZF5A-10，ZF5A-11，ZF5A-15) possessed an additional cysteine residue in the unique domain ．Based on the occurrence of the four major T cell immunogenic peptides and glutamine residues in the glutamine repeats，ZF5A- 7and ZF5A-12 could be assigned to chromosome 6A，ZF5A-4,ZF5A-13，ZF5A-14 and ZF5A-17 to chromosome 6B，and other 11 genes to chromosome 6D．The sec- ondary structure predication of 17 cloned genes and other four genes in public database demonstrated that the num- bers and distributions of-helix and-strand were variable in different mature-gliadins．Generally，5- helixes (H₁－H₅) usually presented in the two glutamine repeats and two unique domains for majority of- gliadins，but theputative proteins of ZF5A-17 lost the- helix(H₂ )，ZF5A-2 and ZF5A-8 had an additional-helix in the uni domain (H_E1 )，and GQ891685 and ZF5A- 15 had an additional- helix in the second glutamine repeat(H_E2 ) addition,most of the genes(61．11%) had a-strand(E) in the C-terminal unique domain ．High variations numbers of cysteine residue,-helixes and-strands in the-gliadins of Zhengfeng 5 might be strongly related its good rheological properties of dough．

Key words: α- gliadin, Zhengfeng 5, Gene clonging, Sequence analysis

CLC Number:

S512.1
Q78

LI Yu-ge, XING Ran-ran, CUI Yi-fei, LI Suo-ping. Cloning and Sequencing of－ gliadin Genes from Common Wheat Cultivar Zhengfeng 5[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 46-52. doi: 10.3969/j.issn.1000-7091.2013.04.009.

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