Gene Cloning and Sequence Analysis of SuSy Gene in Peanut （ Arachis hypogaea L. ）

doi:10.3969/j.issn.1000-7091.2012.04.005

Abstract

Abstract: Sucrose Synthase （SuSy）,a key enzyme in sucrose metabolism, plays very role in both plant growth and developmental process. It is reported that SuSy gene is also closely relate to the ability of plant resistance to abiosmotic stress. In order to reveal the mechanism of SuSy gene in peanut, here, the TAIL-PCR technology of genome walking was used to obtain a genome sequence for the length of 6 189 bp,which included 13 exons, 12 introns and about an 825 bp length partial promoter region. Sequence analysis showed that typical elements as TATA-box, CAAT-box and some other regulate elements were contained in this region, such as cis-acting element involved in low temperature responsive element （LTR）, GA responsive element,drought responsive of MYB hinging site, anae-robic induction element ARE and light responsive element G-box and box4 were found in the sequence. It was sup- posed that the expression of SuSy gene was induced by low temperature,drought,anaerobic and hormone GA etc.

Key words: Peanut, SuSy gene, Gene cloning, Promoter, Genome walking

CLC Number:

HE Mei-jing, LIU Li-feng, MU Guo-jun, HOU Ming-yu, CHEN Huan-ying, CUI Shun-li. Gene Cloning and Sequence Analysis of SuSy Gene in Peanut （ Arachis hypogaea L. ）[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 23-28. doi: 10.3969/j.issn.1000-7091.2012.04.005.

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References

[1] Jang J C, Leon P, Zhou L, et al. Hexokinase as a sugar sensor in higher plants [J]. Plant Cell, 1997,9( 1 ) :5 - 19.

[2] Loreti E,Bellis L D,Alpi A,et al. Why and how do plant cells sense sugars [J]. Ann Bot,2001,88 :803 - 812.

[3] Hanson H D, Hitz W D. Metabolic responses of meso- phytes to plant water deficits [J]. Annu Rev Plant Physi- ol, 1982,33 : 163 - 203.

[4] Kramer P J, Boyer J S. Water Relations of Plants and Soils [M]. San Diego : Academic Press, 1995:377 - 404.

[5] Prado F E, Boero C, Gallardo M,et al. Effect of NaCl on germination,growth,and soluble sugar content in Chenop- odium quinoa wild seeds [J]. Bot Bull Acad Sin,2000, 41:27 -34.

[6] Calderon P, Pontis H G. Increase of sucrose synthase ac- tivity in wheat plants after a chilling shock [J]. Plant Sci, 1985,42 : 173 - 176.

[7] D jardin A,Sokolov L N, Kleczkowski L A. Sugar/osmoti- cum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis [J]. Biochem, 1999,344:503 - 509.

[8] Baud S, Vauhier M N, Rochat C. Structure and expression profile of the sucrose synthase multigene family in Arabi- dopsis [J]. J Exp Bot,2004,55:397 - 409.

[9] Fujii S, Hayashi T, Mizuno K. Sucrose synthase is an inte- gral component of the cellulose synthesis machinery [J]. Plant Cell Physiol,2010,51 (2) :294 - 301.

[10] Coleman H D,Yan J, Mansfield S D. Sucrose synthase affects carbon partitioning to increase cellulose produc- tion and altered cell wall ultrastructure [J]. Proc Natl Acad Sci USA,2009,4(31) :13118 - 13123.

[11] Brill E,Thournout M, White R G,et al. A novel isoform of sucrose synthase is targeted to the cell wall during secondary cell wall synthesis in cotton fiber [J]. Plant Physiol,2011,157 ( 1 ) :40 - 54.

[12] Fern ndez E B,Munoz F J,Li J,et al. Sucrose synthase activity in the susl/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch produc- tion [J]. Proc Natl Acad Sci USA, 2012,109 ( 1 ) : 321 - 326.

[13] Geiser M, Doring H P, Wostemeyer J, et al. A cDNA clone from Zea mays endosperm sucrose synthetase Mr- na [J]. Nucleic AcidsRes,1980,8(24) :6175 -6188.

[14] Buchner P,Poret M, Rochat C. Cloning and characteriza- tion of a cDNA encoding a second sucrose synthase gene in pea ( Pisum sativum L. ) [J]. Plant Physiol, 1998, 117:719.

[15] Wienkoop S, Larrainzar E, Glinski M, et al. Absolute quantification of Medicago truncatulasucrose synthase isoforms and N-metabolism enzymes in symbiotic root nodules and the detection of novel nodule phosphopro- reins by mass spectrometry [J]. J Exp Bot, 2008,59 (12) :3307 -3315.

[16] Chopra S, Del-favero J, Dolferus R, et al. Sucrose syn- thase of Arabidopsis: Genomic cloning and sequence characterization [J]. Plant Mol Biol, 1992,18:131 - 134.

[17] Sturm A, Tang G Q. The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning [J]. Trends Plant Sci, 1999,4:401 - 407.

[18] Komatsu A, Moriguchi T, Koyama K,et al. Analysis of sucrose synthase genes in citrus suggests different roles and phylogenetic relationships [J]. J Exp Bot,2002,53 (366) :61 -71.

[19] Crespi M D,Zabaleta E J, Pontis H G, et al. Sucrose syn- thase expression during cold acclimation in wheat [J]. Plant Physiol, 1991,96:887 - 891.

[20] Rosa M, Hilal M, Gonz lezb effect on enzyme activities J A, et al. Low-temperature involved in sucrose-starch partitioning in salt-stressed and sah-acclimated cotyle- dons of quinoa ( Chenopodium quinoa Willd. ) seedlings [J]. Plant Physiol Bioch,2009,47 ( 4 ) : 300 - 307.

[21] Kleines M, Elster R C, Rodrigo M J,et al. Isolation and expression analysis of two stress-responsive sucrose-syn- thase genes from the resurrection plant Craterostigma plantagineum ( Hochst. ) [J]. Planta, 1999,209 : 13 - 24.

[22] Sicilia C B, Amado S H, Melendi G P,et al. Structure, expression profile and subcellular localisation of four dif- ferent sucrose synthase genes from barley [J]. Planta, 2011,234(2) :391 -403.

[23] Niedzwiedz-Siegiena, Bogatek-Leszczynskaa R, C mea D,et al. Effects of drying rate on dehydration sensitivity of excised wheat seedling shoots as related to sucrose metabolism and antioxidant enzyme activities [J]. Plant Sci, 2004,167 (4) : 879 - 888.

[24] Lu S W, Li T L, Jiang J. Effects of salinity on sucrose metabolism during tomato fruit development [J]. J Aft B iotechnol, 2010,9 ( 6 ) :842 - 849.

[25] Arrese-Igor C, Gonzalez E M, Gordon A J,et al. Sucrose synthase and nodule nitrogen fixation under drought and other environmental stresses [J]. Plant Physiol, 1999, 27:189 -212.

[26] Silvente S, Camas A, Lara M. Heterogeneity of sucrose synthase genes in bean ( Phaseolus vulgaris L. ): evi- dence for a nodule-enhanced sucrose synthase gene [J].J Exp Bot,2003,54 (383):749 -755.

[27] Salanoubat M, Belliard G. The steady-state level of potato sucrose synthase mRNA is dependent on wounding, anaerobiosis and sucrose concentration [J]. Gene, 1989, 84(1) :181 - 185.

[28] Zeng Y,Wu Y,Avigne W T,et al. Differential regulation of sugar-sensitive sucrose synthases by hypoxia and an- oxia indicate complementary transcriptional and posttran- scriptional responses [J]. Plant Physiol, 1998,116 : 1573 - 1583.

[29] Loreti E, Poggi A, Novi G,et al. A genome-wide analysis of the effects of sucrose on gene expression in Arabidop- sis seedlings under anoxia [J]. Plant Physiol, 2005, 137:1130 -1138.

[30] Hardin S C, Huber S C. Proteasome activity and the post- translational control of sucrose synthase stability in maize leaves [J]. Plant Physiol Bioch,2004,42 : 197 - 208.

[31] Sch fer W E, Rohwer J M, Botha F C. Partial purification and characterisation of sucrose synthase in sugarcane [J]. Plant Physiol,2005,162( 1 ) : 11 - 20.

[32] Sturm A, Lienhard S, Schatt S, et al. Tissue-specific ex- pression of two genes for sucrose synthase in carrot (Daucus carota L. ) [J]. Plant Mol Biol, 1999,39:349 - 360.

[33] Bieniawska Z, Paul Barratt D H, Garlick A P, et al. A- nalysis of the sucrose synthase gene family in Arabidopsis [J]. J Plant,2007,49,810 - 828.

[34] Lingle S E,Dyer J M. Polymorphism in the promoter re- gion of the sucrose synthase - 2 gene of saccharum geno- types[J]. American Society Sugar Cane Technologists, 2004,24:241 - 249.

[35] Breathnach R, Chambon P. Organization and expression of eukaryotic split genes coding for proteins [J]. Ann Rev Biochem, 1981,50:349 - 383.

[36] Liu Y G, Whittier R F. Thermal asymmetric interlaced PCR:automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking [J]. Genomics, 1995,25 ( 3 ) :674 - 681.

[37] Dunn M A,Whitel A J,Vural S,et al. Identification of promoter elements in a low-temperature-responsive gene ( bit4. 9) from barley ( Hordeum vulgare L. ) [J]. Plant Mol Biol, 1998,38 : 551 - 564.

[38] Yamaguchi-Shinozaki K,Shinozaki K. A novel cis-acting element in all Arabidopsis gene is involved in responsive- ness to drought,low-temperature, or high-sah stress [J]. Plant Cell, 1994,6:251 - 264.

[39] Riou C, Herv C, Pacquit V, Dabos P,et al. Expression of an Arabidopsis lectin kinase receptor gene, lecRK-al, is induced during senescence, wounding and in response to oligogalacturonic acids [J]. Plant Physiol Biochem, 2002,40:431.

[40] Mena M, CeJudo F J, Isabel-Lamoneda I,et al. A role for the DOF transcription factor BPBF in the regulation of gibberellin-responsive genes in barley aleurone [J]. Plant Physiol, 2002,130 ( 1 ) : 111 - 119.

[41] Zou M, Guan Y, Ren H,Zhang F,et al. A bZIP tran- scription factor,OsABI5,is involved in rice fertility and stress tolerance [J]. Plant Mol Biol, 2008,66 ( 6 ) : 675 -683.

[42] MillarA J, Kay S A. Integration of circadian and photo transduction pathways in the network controlling CAB gene transcription in Arabidopsis [J]. Proc Nat Acad. 1996,93 : 15491 - 15496.

[43] Daraselia N D,Tarchevskaya S, Narita J O. The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme a reduc- tase gene 2 has unusual regulatory elements that direct high-level expression [J]. Plant Physiol, 1996,112 ( 2 ) : 727 -733.

[44] Hirose T, Scofield G N,Terao T. An expression analysis profile for the entire sucrose synthase gene family in rice [J]. Plant Sci,2008,174(5) :534 -543.

[45] Zhang D Q,Xu B H,Yang X H,et al. The sucrose syn- thase gene family in Populus : structure, expression, and evolution [J]. Tree Genet Genom, 2011,7 ( 3 ) : 443 - 456.

[46] 《花生加工利用、贸易现状与展望》[J].武汉工业学院学报,2002(2).

[47] 《改良CTAB法和高盐低pH值法提取花生DNA的效果》[J].花生学报,2002(3).

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