Abstract: F₁ hybrids were obtained by crossing PEPC transgenic rice germplasm as male parent with Japonica rice cultivar 9516. Processed with the cultivation of pollen lines, selection by photosynthetic characteristic and enzyme activity, and systematic reselection and re-identification of three generations, stable JAAS45 pollen lines of pure-bred diploid plants were achieved. The content of photosynthetic pigment, net photosynthetic rate and water use efficiency of JAAS45 and its parents were investigated in this paper. After tillering stage chlorophyll contents were higher than 9516, and retained a much higher level close to PC. From seeding to heading period the chla/b ratio of JAAS45and PC were higher than that's of 9516. The net photosynthetic rate(Pn) of JAAS45 approximated that of 9516 at the seeding stage, but it exceeded 9516 at tillering stage, which was even higher than PC at heading and filling stage. From seeding to heading period, water use efficiency of for JAAS45 was evidently higher than 9516, whereas close to PC. Theses results suggested that the photosynthetic characteristics of JAAS45 exhibited higher photosynthetic capacity and water use efficiency, which was similar with that's of the male parent PC. By crossing PEPC transgenic rice germplasm as male parent with the hybrid male of japonica rice, the PEPC transgenic rice can be utilized in transferring the maize PEPC gene into the common rice genome and also useful in the breeding of new rice cultivars with high photosynthetic efficiency and high yield.

Key words: Rice, Phosphoenolpyruvate carboxylase(PEPC) gene, Net photosynthetic rate, Water use efficiency

CLC Number: 

• S511.01