Response of Protein in Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA

doi:10.3321/j.issn:1000-7091.2002.03.009

Abstract

Abstract: Physiological and biochemical response of the embryogenic calluses of sand reed (SREC)and water reed(WREC)to PEG-6000 osmotic stress and exogenous ABA was studied.The result showed that the ability of osmotic stress resistance of SREC was obviously greater than that of WREC while the decrease of soluble protein in WREC was more than that in SREC.The response of WREC to exogenous ABA(10 μmol/ L)was much more sensitive than that of SREC.SDS-PAGE analysis showed a new 17 kD protein band when SREC was treated with 30 %PEG-6000 osmotic stress or when SREC and WREC was treated with 30% PEG-6000 osmotic stress and exogenous ABA simultaneously.

Key words: Reed ecotypes, Embryogenic calluse, Osmotic stress, ABA, Protein

CLC Number:

S564+.2

CHEN Long, LIU Huaipan, ZHANG Chenglie. Response of Protein in Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(3): 41-45. doi: 10.3321/j.issn:1000-7091.2002.03.009.

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