Some Physiological Response of the Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA

doi:10.3321/j.issn:1000-7091.2001.03.008

Abstract

Abstract: We've studied the physiological changes of the embryogenic calluses of sand reed(SREC) and the water reed(WREC) in response to the osmotic stress of PEG 6000 and exogenous ABA.The results showed that the contents of free proline,relative membrane permeability and safeguard enzyme(CAT,POD and SOD)activity of SREC and WREC increased under the PEG osmotic stress treatment from 10%~30%,while the relative water content(RWC) decreased under the same treatment.Still the contents of proline and safeguard enzyme activity of SREC increased higher than these of WREC While the relative membrane permeability and RWC of SREC decreased less than these of WREC.It also showed the response of WREC to exogenous ABA was much higher than that of SREC.

Key words: Reed ecotypes, Enbryogenic callus, Osmotic stress, Exogenous ABA, Physiological andbiochemical response

CLC Number:

S64+.2

WU Shiguang, CHEN Long, LIU Huaipan, ZHANG Chenglie. Some Physiological Response of the Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 35-39. doi: 10.3321/j.issn:1000-7091.2001.03.008.

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