Determination of β-carotene in Different Pumpkin Varieties by HPLC

doi:10.3321/j.issn:1000-7091.1998.03.028

Abstract

Abstract: β-carotene from pulps of 31 varieties of pumpkin were determined by reversed phase high performance liquid chromatography （RP HPLC） with ultraviolet （UV） detector（254nm）,a C18 column and an eluant of 90% methanol and ethyl acetate.Dried powder samples were extracted by petroleum ether,evaporated to dryness and then dissolved by hexane to a final ratio of 1g of dried sample per 10ml hexane.The hexane solutions were analysed by HPLC.Difference of contents of β carotene between pumpkin varieties were found significant.Content of β carotene in spanish pumpkin was 8 735mg/100g .F.W.,which was far higher than that of other pumpkin varieties and even higher than the content of β carotene in carrot.Spanish pumpkin can be considered as a β carotene rich vegetable.

Key words: &beta, carotene, Pumpkin, RP HPLC

CLC Number:

S642.1

Wu Zengru， Jin Tongming. Determination of β-carotene in Different Pumpkin Varieties by HPLC[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(3): 141-144. doi: 10.3321/j.issn:1000-7091.1998.03.028.

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