Cotton Genetic Transformation and Plant Regeneration

doi:10.3321/j.issn:1000-7091.1997.02.016

Abstract

Abstract: Hypocotyl segments from 5 6 day old seedling of Gossypium hirsutum were co-cultured with Agrobacterium tumefacience,GUS gene as marker gene,NPT as selecting gene.After the cultures were co cultured in MS medium contained 0.1 mg/L 2,4 D and 0 1mg/L KT for 48h,then they were transferred into the medium described above suplemented with 500mg/l cefotaxime and 50 100mg/L kanamycin to induce and select resistant calli.After 70 80d the induction frequence of calli was calculated.The expression of Gus gene was tested with histochemistry location method and the transformation frequency was recorded.The experimental result indicated that the average rate of callus was 40% or so, and the highest was over 60%.The average rate of callus transformation was 30% or so,and the highest was 70%.The frequency of regenerated plant transformation was 30% or so,and the highest was over 60%.According to the result of DNA molecular hybridization,the presence of introduced gene in the genome of the transgenic plants was confirmed. It was also confirmed that BT gene had been integrated into genome of transgenic plants and was inherited into its progenies.

Key words: Cotton, Hypocotyl, Callus rate, Transformation rate

CLC Number:

S562.035

Jiao Gaili, Guo Sandui, Li Shujun, Chen Zhixian, Zhao Junxia. Cotton Genetic Transformation and Plant Regeneration[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1997, 12(2): 82-85. doi: 10.3321/j.issn:1000-7091.1997.02.016.

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