摘要：

旨在研究酿酒葡萄渣对氧化应激肉鸡的生长性能、抗氧化指标及相关基因表达量的影响。将120只体质量相近、健康的30日龄良凤肉公鸡,随机分为5组,即阴性对照组(NC组,饲喂基础饲粮)、阳性对照组(地塞米松(Dex)组,饲喂基础饲粮)、Dex+1%葡萄渣组、Dex+2%葡萄渣组、Dex+4%葡萄渣组,每组4个重复。7周龄时,阳性对照组和试验组腹腔皮下注射Dex诱导氧化应激模型,NC组注射等剂量生理盐水,每隔1 d注射1次,每周3次,注射7 d。试验期35 d。结果发现:酿酒葡萄渣能显著提高Dex诱导氧化应激肉鸡日平均采食量;能够显著提高肉鸡血清免疫球蛋白M活性;能够显著提高超氧化物歧化酶、谷胱甘肽过氧化物酶、总抗氧化能、过氧化氢酶含量;能够显著提高超氧化物歧化酶、谷胱甘肽过氧化物酶mRNA相对表达量。由此可见,酿酒葡萄渣能提高肉鸡免疫能力和抗氧化能力,有效缓解Dex诱导氧化应激状态下肉仔鸡的应激反应。

关键词: 肉鸡, 葡萄渣, 氧化应激, 抗氧化指标, 生长性能

Abstract:

This experiment aimed to investigate the effects of grape residue on the growth performance,antioxidant indicator,and related gene expression in broilers under oxidative stress.One hundred and twenty healthy 30-day-old Liangfeng broiler roosters with similar body weights were randomly divided into five groups:a negative control group (NC group,fed a basal diet),a positive control group (Dex group,fed a basal diet),a Dex+1% grape pomace group,a Dex+2% grape pomace group,and a Dex+4% grape pomace group,with four replicates per group.At 7 weeks of age,the positive control group and experimental group were injected with Dex to induce an oxidative stress model,while the NC group received an equal dose of normal saline.For one week,injections were administered three times a week,every other day.The experiment lasted for 35 days.The results showed that winemaking grape pomace significantly increased the ADFI of Dex-induced oxidative stress broilers;significantly enhanced serum IgM activity of broilers;significantly increased the levels of superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and catalase; and significantly upregulated the relative mRNA expression of superoxide dismutase and glutathione peroxidase. In conclusion,wine grape residue can enhance broilers' immune capacity and antioxidant capacity,and effectively alleviate the stress response of broilers under Dex-induced oxidative stress.

Key words: Broiler, Grape residue, Oxidative stress, Antioxidant index, Growth performance

中图分类号: 

• S831.5