Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析

doi:10.3969/j.issn.1000-7091.2012.05.017

华北农学报 ›› 2012, Vol. 27 ›› Issue (5): 91-95. doi: 10.3969/j.issn.1000-7091.2012.05.017

所属专题： 畜牧；生物技术；

Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析

刘桂芬¹, 万发春¹, 刘桂萍², 刘晓牧¹, 逯茂洋³, 谭秀文¹, 成海建¹, 宋恩亮¹

山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室;
新疆大学地质与矿业工程学院;
中国农业大学动物医学院

收稿日期:2012-07-20 出版日期:2012-10-28
通讯作者: 宋恩亮(1971-)，男，山东兖州人，副研究员，硕士，主要从事肉牛育种与疾病防治的研究。
作者简介:刘桂芬(1980-)，女，山东东明人，助理研究员，博士，主要从事动物分子遗传研究。
基金资助:
山东省生物基因资源发掘与种质创新利用研究项目;2010年山东省优秀中青年科学家科研奖励基金(BS2010SW026);3-羟-3-甲基辅酶A还原酶对渤海黑牛胆固醇合成的影响研究(31100890);山东省自主创新重大科技专项计划(2011ZHZX1A0406);“十二五”农村领域国家科技计划课题(2011BAD28B04-02)

Associations of PCR-RFLP in Pit-1/Hinf Ⅰ Gene with Growth Traits in Hybrid Cattle of Luxi Yellow Cattle and Limousin

LIU Gui-fen¹, WAN Fa-chun¹, LIU Gui-ping², LIU Xiao-mu¹, LU Mao-yang³, TAN Xiu-wen¹, CHENG Hai-jian¹, SONG En-liang¹

1. Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Shandong Key Lab of Animal Disease Control and Breeding Jinan 250100 China;

2. College of Geology and Mineral Engineering of Xinjiang University Urmuqi 830046 China ;
3. College of Veterinary Medicine China Agricultural University Beijing 100193 China

Received:2012-07-20 Published:2012-10-28

摘要/Abstract

摘要： 为了提高鲁西黄牛生长发育水平及产肉性能。利用PCR-RFLP技术研究111头利鲁杂交牛Pit-1基因多态性与体尺生长性状指标之间的相关性。结果表明,群体的Pit-1-HinfⅠ基因座的451 bp的PCR产物被限制性内切酶HinfⅠ酶切后表现多态性,它们的等位基因A、B频率分别为0.32,0.68,不符合Hardy-Weinberg平衡状态。群体Pit-1基因座不同基因型与体尺生长性状指标相关分析的结果表明,群体内AA基因型个体的尻高显著高于群体AB和BB型个体(P<0.01),可作为利鲁杂交牛尻高的候选基因之一;群体内AB基因型个体的髋宽显著高于群体AA和BB型个体(P<0.05);但在头长、额宽、胸围、胸深、胸宽、体斜长、尻长、腰角宽、坐骨结节宽、管围、体高、十字部高、臀端高等指标上均无显著差异(P>0.05)。

关键词: Pit-1, PCR-RFLP, 生长性能, 利鲁杂交牛

Abstract: Improving the grouth level and meat production of Luxi Yellow Cattle,this paper use PCR-RFLP technology to analysis the correlation of Pit-1 gene polymorphism and growth traits of 111 hybrid cattle of Luxi Yellow Cattle and Limousin.The results show that the Pit-1-HinfⅠ loci of 451 bp′s PCR products is polymorphism after enzymes Hinf Ⅰ enzyme,the allele frequency of A and B were 0.32 and 0.68,it is not fit for Hardy-Weinberg balance.The rump length of AA genotype is higher than AB and BB genotype within population(P0.01),it can be used one of candidate genes;The hip width of AB genotype is higher than AA and BB genotype(P0.05) within the group;But there are no differences among 3 genotypes in the head length,forehead width,chest circumference,chest deep and wide,body length,rump length,waist angular wide,shank circumference,body height,the cross department high,the hip higher(P0.05)

Key words: Pit-1, PCR-RFLP, Growth traits, Hybrid cattle of Lilu

中图分类号:

S823

刘桂芬, 万发春, 刘桂萍, 刘晓牧, 逯茂洋, 谭秀文, 成海建, 宋恩亮. Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析[J]. 华北农学报, 2012, 27(5): 91-95. doi: 10.3969/j.issn.1000-7091.2012.05.017.

LIU Gui-fen, WAN Fa-chun, LIU Gui-ping, LIU Xiao-mu, LU Mao-yang, TAN Xiu-wen, CHENG Hai-jian, SONG En-liang. Associations of PCR-RFLP in Pit-1/Hinf Ⅰ Gene with Growth Traits in Hybrid Cattle of Luxi Yellow Cattle and Limousin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 91-95. doi: 10.3969/j.issn.1000-7091.2012.05.017.

http://www.hbnxb.net/CN/Y2012/V27/I5/91

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Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析

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Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析

Associations of PCR-RFLP in Pit-1/Hinf Ⅰ Gene with Growth Traits in Hybrid Cattle of Luxi Yellow Cattle and Limousin

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