温氏土鸡和白洛克鸡胚小肠PepT1和NHEs mRNA表达差异及发育性变化

doi:10.3969/j.issn.1000-7091.2012.05.018

华北农学报 ›› 2012, Vol. 27 ›› Issue (5): 96-100. doi: 10.3969/j.issn.1000-7091.2012.05.018

所属专题： 畜牧；

温氏土鸡和白洛克鸡胚小肠PepT1和NHEs mRNA表达差异及发育性变化

黎相广, 严会超, 曾佩玲, 束刚, 张德祥, 罗庆斌, 王修启

华南农业大学动物科学学院

收稿日期:2012-07-17 出版日期:2012-10-28
通讯作者: 王修启(1968-)，男，河南新乡人，研究员，博士，主要从事畜禽营养研究。
作者简介:黎相广(1987-)，男，广东湛江人，在读硕士，主要从事家禽分子营养研究。严会超为同等贡献作者。
基金资助:
NSFC-广东联合基金(U0931004);公益性行业(农业)科研专项(201003011)

The Ontogenetic Expression of PepT1 and NHEs mRNA in the Small Intestine of Broiler Embryos

LI Xiang-guang, YAN Hui-chao, ZENG Pei-ling, SHU Gang, ZHANG De-xiang, LUO Qing-bin, WANG Xiu-qi

College of Animal Science South China Agricultural University Guangzhou 510642 China

Received:2012-07-17 Published:2012-10-28

摘要/Abstract

摘要： 为研究温氏土鸡和白洛克鸡胚小肠寡肽转运载体PepT1(Solute carrier family 15 member 1)以及Na+/H+交换载体NHE2(Solute carrier family 9 member 2)和NHE3(Solute carrier family 9 member 3)mRNA的表达规律,选取产蛋日龄相近的温氏土鸡和白洛克鸡所产种蛋各96枚,每个品种随机分为6组,每组16枚,在相同的条件下进行孵化。分别在9,12,14,17,19胚龄(E)和出壳当天(DOH),每个品种选取16枚鸡胚,采集小肠样品,采用Real-time RT-PCR方法检测小肠PepT1、NHE2和NHE3 mRNA的相对表达丰度。结果显示:从发育性变化来看,PepT1、NHE2和NHE3mRNA在温氏土鸡和白洛克鸡胚小肠的表达丰度都表现为随着胚龄的增加而上调,19胚龄后上调幅度较大;双因素方差分析表明,PepT1、NHE2和NHE3 mRNA的表达丰度在2个品种间无显著差异,但受发育阶段影响,PepT1 mRNA的表达丰度存在"品种×胚龄"的互作效应(P<0.000 1),NHE2和NHE3 mRNA表达丰度无"品种×胚龄"的互作效应(P>0.05)。说明PepT1、NHE2和NHE3 mRNA表达丰度在温氏土鸡和白洛克鸡胚小肠中具有相同的发育模式,其表达量受发育水平的影响,但品种间差异不显著。

关键词: PepT1, NHE2, NHE3, 表达规律, 鸡胚, 小肠

Abstract: The objective of this study was to evaluate the difference of PepT1(solute carrier family 15 member 1),NHE2(solute carrier family 9 member 2) and NHE3(solute carrier family 9 member 3) mRNA abundance in the small intestine of broiler embryos.A total of 192 hatching eggs produced by White Plymouth Rock(WPR) and WENS Yellow Feather Chicken(WYFC) broiler breeders with similar breeding age were study.Hatching eggs of both breeds were incubated in the same incubator.Each of 16 small intestine samples were taken on embryonic day(E) 9,12,14,17,19 and the day of hatch(DOH).the relative abundance of PepT1,NHE2 and NHE3 mRNA was measured by real-time RT-PCR method and was normalized by GAPDH(glyceraldehyde-3-phosphate dehydrogenase) mRNA abundance.The results indicated that：the relative abundance of PepT1,NHE2 and NHE3 mRNA grew up with the development of chick embryos and that the faster growth rates appeared posterior E19;the abundance of PepT1,NHE2 and NEH3 mRNA was not affected by breed,but affected by embryonic day;there was an interaction of breed-embryonic day on PepT1 mRNA abundance(P0.000 1),but no on the mRNA abundance of NHE2 and NHE3(P0.05).We concluded that the abundance of PepT1,NHE2 and NHE3 mRNA shared the same expression pattern in the small intestine of both WYFC and WPR embryos and was regulated by development of embryo but not by breed.

Key words: PepT1, NHE2 NHE3 mRNA abundance, Chick embryo, Small intestine

中图分类号:

S831

黎相广, 严会超, 曾佩玲, 束刚, 张德祥, 罗庆斌, 王修启. 温氏土鸡和白洛克鸡胚小肠PepT1和NHEs mRNA表达差异及发育性变化[J]. 华北农学报, 2012, 27(5): 96-100. doi: 10.3969/j.issn.1000-7091.2012.05.018.

LI Xiang-guang, YAN Hui-chao, ZENG Pei-ling, SHU Gang, ZHANG De-xiang, LUO Qing-bin, WANG Xiu-qi. The Ontogenetic Expression of PepT1 and NHEs mRNA in the Small Intestine of Broiler Embryos[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 96-100. doi: 10.3969/j.issn.1000-7091.2012.05.018.

http://www.hbnxb.net/CN/Y2012/V27/I5/96

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温氏土鸡和白洛克鸡胚小肠PepT1和NHEs mRNA表达差异及发育性变化

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温氏土鸡和白洛克鸡胚小肠PepT1和NHEs mRNA表达差异及发育性变化

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