纳米硒对热应激虹鳟血清抗氧化指标和免疫指标的影响

doi:10.7668/hbnxb.20192837

摘要/Abstract

摘要：

为研究纳米硒对热应激下虹鳟血清抗氧化指标和免疫指标的影响,选取平均体质量约80 g的8月龄虹鳟600尾,随机分为3组,其中,对照组饲喂基础日粮,处理组分别饲喂添加了5,10 mg/kg纳米硒的日粮,在虹鳟适宜生长水温(18 ℃)条件下饲喂9 d在24 ℃ (虹鳟产生热应激)下热应激48 h;分别在热应激前(18 ℃,0,3,6,9 d)和热应激(24 ℃,4,8,12,24,48 h)每组随机取10尾鱼,采集血清,测定抗氧化和免疫指标。结果发现,相比对照组添加纳米硒显著提高了血清总蛋白(TP)、白蛋白(ALB)和球蛋白(GLB)含量;热应激下谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的含量降低,过氧化氢酶(CAT)和丙二醛(MDA)的含量升高,纳米硒提高了GSH-Px、SOD和CAT含量,降低了MDA含量;热应激使对照组血清肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)的含量逐渐升高,5 mg/kg纳米硒组在热应激4 h相比对照组TNF-α和IL-1β的含量显著提高,热应激12 h相比对照组IL-1β的含量显著降低;免疫球蛋白M(IgM)在热应激4 h含量下降,热应激12 h含量上升,补体3(Complement 3,C3)的含量在热应激下逐渐下降,纳米硒则显著提高了IgM和C3的含量。综上,饲粮中添加纳米硒可提高热应激下虹鳟血清抗氧化和免疫指标,且相比10 mg/kg添加量,添加5 mg/kg纳米硒对机体的保护能力更强。

关键词: 虹鳟, 纳米硒, 热应激, 抗氧化指标, 免疫指标

Abstract:

In order to study the effects of nano-selenium on serum antioxidant and immune indexes of rainbow trout under heat stress,600 8-month-old rainbow trout with an average body weight of 80 g were selected and randomly divided into 3 groups:the control group was fed with the basal diet,and the experimental groups were fed with 5,10 mg/kg nano-selenium diets.The rainbow trout was fed at the suitable growth water temperature(18 ℃)for 9 days,At 24 ℃ (heat stress for rainbow trout)for 48 h.10 fish were randomly selected from each group before heat stress(18 ℃,0,3,6,9 d)and after heat stress(24 ℃,4,8,12,24,48 h)and serum was collected to determine antioxidant and immune indexes.The results showed that compared with the control group,the experimental group significantly increased the contents of TP,ALB and GLB in serum(P<0.05);the activity of GSH-Px and SOD were significantly decreased,as well as CAT and MDA were significantly increased in heat stress,but the addition of nano-selenium significantly increased the activity of GSH-Px,SOD,CAT and decreased MDA activity;the contents of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in serum of the control group were gradually increased by heat stress. The contents of TNF-α and IL-1β in serum of the 5 mg/kg nano-selenium group were significantly increased at 4 h, and the contents of IL-1β were significantly decreased at 12 h compared with the control group.The content of IgM decreased at 4 h and increased at 12 h after heat stress,and nano-selenium supplementation significantly increased the activity of IgM;after heat stress,the content of C3 decreased gradually,but the addition of nano-selenium significantly increased the activity of C3.In conclusion,dietary nano-selenium supplementation can improve the antioxidant capacity and immune capacity of rainbow trout under heat stress.Compared with 10 mg/kg nano-selenium,the antioxidant effect and immune effect of 5 mg/kg nano-selenium are more stable.

Key words: Rainbow trout, Nano-selenium, Heat stress, Antioxidant index, Immune index

卢军浩, 李兰兰, 权金强, 赵桂研, 孙军, 林桥荣, 刘哲. 纳米硒对热应激虹鳟血清抗氧化指标和免疫指标的影响[J]. 华北农学报, 2022, 37(3): 231-238. doi: 10.7668/hbnxb.20192837.

LU Junhao, LI Lanlan, QUAN Jinqiang, ZHAO Guiyan, SUN Jun, LIN Qiaorong, LIU Zhe. Effects of Nano-selenium on Serum Antioxidant and Immune Indexes of Oncorhynchus mykiss under Heat Stress[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 231-238. doi: 10.7668/hbnxb.20192837.

http://www.hbnxb.net/CN/Y2022/V37/I3/231

图/表 4

表1 饲料中硒元素含量测定

Tab.1 Determination results of selenium content in feedmg/kg

编号 Sample	0 mg/kg			5 mg/kg			10 mg/kg
编号 Sample	1	2	3	1	2	3	1	2	3
硒含量 Selenium content	0.44	0.66	0.59	5.75	5.74	5.66	10.07	10.53	10.63

图1 纳米硒对热应激下虹鳟血清蛋白含量的影响(n=10) 不同小写字母表示同一时间点下不同处理组间酶活性差异显著(P<0.05)。图2,3同。

Fig 1. Effects of nano-selenium on serum protein content of rainbow trout under heat stress(n=10) Different lowercase letters indicate significant difference of enzyme activity between different treatment groups at the same time(P<0.05).The same as Fig.2,3.

图2 纳米硒对热应激下虹鳟血清抗氧化酶的影响(n=10)

Fig.2 Effects of nano-selenium on antioxidant enzymes in serum of rainbow trout under heat stress(n=10)

图3 纳米硒对热应激下虹鳟血清免疫相关酶的影响(n=10)

Fig.3 Effects of nano-selenium on immune related enzymes in rainbow trout serum under heat stress(n=10)

参考文献 36

[1]	刘哲, 李勤慎, 蔡原, 王建福, 黄进强, 柴文琼, 康鹏天. 3种虹鳟养殖群体的遗传结构及遗传多样性分析[J]. 水生态学杂志, 2010, 31(2):48-53.doi: 10.15928/j.1674-3075.2010.02.022. doi: 10.15928/j.1674-3075.2010.02.022
	Liu Z, Li Q S, Cai Y, Wang J F, Huang J Q, Chai W Q, Kang P T. Study on genetic structure and genetic diversity of three populations of trout(Oncorhynchus mykiss)[J]. Journal of Hydroecology, 2010, 31(2):48-53.
[2]	夏斌鹏, 刘哲, 周彦静, 王永杰, 黄进强, 李永娟, 康玉军, 王建福, 刘晓霞. 慢性热应激对虹鳟部分血清非特异性免疫指标的影响[J]. 农业生物技术学报, 2017, 25(7):1078-1085.doi: 10.3969/j.issn.1674-7968.2017.07.005. doi: 10.3969/j.issn.1674-7968.2017.07.005
	Xia B P, Liu Z, Zhou Y J, Wang Y J, Huang J Q, Li Y J, Kang Y J, Wang J F, Liu X X. Effects of chronic heat stress on part of serum non-specific immunity parameters in rainbow trout(Oncorhynchus mykiss)[J]. Journal of Agricultural Biotechnology, 2017, 25(7):1078-1085.
[3]	刘晓霞, 刘哲, 王永杰, 康玉军, 马芳, 王建福, 黄进强. 热应激对虹鳟CRT基因和PDIA4基因mRNA表达的影响[J]. 基因组学与应用生物学, 2019, 38(9):3967-3972.doi: 10.13417/j.gab.038.003967. doi: 10.13417/j.gab.038.003967
	Liu X X, Liu Z, Wang Y J, Kang Y J, Ma F, Wang J F, Huang J Q. Effects of heat stress on mRNA expression of CRT and PDIA4 in rainbow trout(Oncorhynchus mykiss)[J]. Genomics and Applied Biology, 2019, 38(9):3967-3972.
[4]	Belhadj Slimen I, Najar T, Ghram A, Dabbebi H, Ben Mrad M, Abdrabbah M. Reactive oxygen species,heat stress and oxidative-induced mitochondrial damage.A review[J]. International Journal of Hyperthermia, 2014, 30(7):513-523.doi: 10.3109/02656736.2014.971446. doi: 10.3109/02656736.2014.971446 pmid: 25354680
[5]	Sheiha A M, Abdelnour S A, Abd El-Hack M E, Khafaga A F, Metwally K A, Ajarem J S, Maodaa S N, Allam A A, El-Saadony M T. Effects of dietary biological or chemical-synthesized nano-selenium supplementation on growing rabbits exposed to thermal stress[J]. Animals:an Open Access Journal from MDPI, 2020, 10(3):430.doi: 10.3390/ani10030430. doi: 10.3390/ani10030430 URL
[6]	Blokhina O, Virolainen E, Fagerstedt K V. Antioxidants,oxidative damage and oxygen deprivation stress:A review[J]. Annals of Botany, 2003, 91(2):179-194.doi: 10.1093/aob/mcf118. doi: 10.1093/aob/mcf118 URL
[7]	Wang J, Wu C S, Hu Y Z, Yang L, Zhang X J, Zhang Y A. Plasmablasts induced by chitosan oligosaccharide secrete natural IgM to enhance the humoral immunity in grass carp[J]. Carbohydrate Polymers, 2022, 281:119073.doi: 10.1016/j.carbpol.2021.119073. doi: 10.1016/j.carbpol.2021.119073 URL
[8]	王志平, 张士璀, 王光锋. 鱼类补体系统成分及补体特异性和功能的研究进展[J]. 水生生物学报, 2008, 32(5):760-769.doi: 10.3724/SP.J.0000.2008.50760. doi: 10.3724/SP.J.0000.2008.50760
	Wang Z P, Zhang S C, Wang G F. Advances on the complement components,characteristic and function of complement system in fish[J]. Acta Hydrobiologica Sinica, 2008, 32(5):760-769. doi: 10.3724/SP.J.1035.2008.00760 URL
[9]	Kohshahi A J, Sourinejad I, Sarkheil M, Johari S A. Dietary cosupplementation with curcumin and different selenium sources(nanoparticulate,organic,and inorganic selenium):Influence on growth performance,body composition,immune responses,and glutathione peroxidase activity of rainbow trout(Oncorhynchus mykiss)[J]. Fish Physiology and Biochemistry, 2019, 45(2):793-804.doi: 10.1007/s10695-018-0585-y. doi: 10.1007/s10695-018-0585-y pmid: 30392099
[10]	Pacitti D, Lawan M M, Sweetman J, Martin S A M, Feldmann J, Secombes C J. Selenium supplementation in fish:A combined chemical and biomolecular study to understand sel-plex assimilation and impact on selenoproteome expression in rainbow trout(Oncorhynchus mykiss)[J]. PLoS One, 2015, 10(5):e0127041.doi: 10.1371/journal.pone.0127041. doi: 10.1371/journal.pone.0127041
[11]	Rotruck J T, Pope A L, Ganther H E, Swanson A B, Hafeman D G, Hoekstra W G. Selenium:biochemical role as a component of glutathione peroxidase[J]. Science, 1973, 179(4073):588-590.doi: 10.1126/science.179.4073.588. doi: 10.1126/science.179.4073.588 pmid: 4686466
[12]	史孟娟. 纳米硒对青鳉鱼的毒性及作用机制研究[D]. 杨凌: 西北农林科技大学, 2017.
	Shi M J. Studies on toxicity and mechanism of Selenium nanoparticles on medaka[D]. Yangling: Northwest A & F University, 2017.
[13]	Yu H B, Zhang C, Zhang X T, Wang C, Li P J, Liu G H, Yan X F, Xiong X F, Zhang L, Hou J Y, Liu S, Zhou J S, Ji H. Dietary nano-selenium enhances antioxidant capacity and hypoxia tolerance of grass carp Ctenopharyngodon idella fed with high-fat diet[J]. Aquacult Nutr, 2020, 26(2):545-557.doi: 10.1111/anu.13016. doi: 10.1111/anu.13016 URL
[14]	Dawood M A O, Zommara M, Eweedah N M, Helal A I, Aboel-Darag M A. The potential role of nano-selenium and vitamin C on the performances of Nile tilapia(Oreochromis niloticus)[J]. Environmental Science and Pollution Research International, 2020, 27(9):9843-9852.doi: 10.1007/s11356-020-07651-5. doi: 10.1007/s11356-020-07651-5 URL
[15]	李彦红, 张飞飞, 黄丽娟, 石艳平, 王依, 廖茂雯, 邬应龙. 纳米硒对齐口裂腹鱼生长、肌肉成分、血清生化及抗氧化指标的影响[J]. 中国水产科学, 2020, 27(6):682-691.doi: 10.3724/SP.J.1118.2020.19307. doi: 10.3724/SP.J.1118.2020.19307
	Li Y H, Zhang F F, Huang L J, Shi Y P, Wang Y, Liao M W, Wu Y L. Influence of dietary nano-selenium on growth,muscle composition,and serum biochemical and antioxidant indices of Schizothorax prenanti[J]. Journal of Fishery Sciences of China, 2020, 27(6):682-691.
[16]	Jiang F, Lin Y, Miao L H, Hao J Y. Addition of bamboo charcoal to selenium(Se)-rich feed improves growth and antioxidant capacity of blunt snout bream(Megalobrama amblycephala)[J]. Animals, 2021, 11(9):2585.doi: 10.3390/ani11092585. doi: 10.3390/ani11092585 URL
[17]	Gao X Q, Fei F, Huo H H, Huang B, Meng X S, Zhang T, Liu B L. Impact of nitrite exposure on plasma biochemical parameters and immune-related responses in Takifugu rubripes[J]. Aquatic Toxicology, 2020, 218:105362.doi: 10.1016/j.aquatox.2019.105362. doi: 10.1016/j.aquatox.2019.105362 URL
[18]	Khan K U, Zuberi A, Fernandes J B K, Ullah I, Sarwar H. An overview of the ongoing insights in selenium research and its role in fish nutrition and fish health[J]. Fish Physiology and Biochemistry, 2017, 43(6):1689-1705.doi: 10.1007/s10695-017-0402-z. doi: 10.1007/s10695-017-0402-z URL
[19]	Shokraneh M, Sadeghi A A, Mousavi S N, Esmaeilkhanian S, Chamani M. Effects of in ovo injection of nano-selenium and nano-zinc oxide and high eggshell temperature during late incubation on antioxidant activity,thyroid and glucocorticoid hormones and some blood metabolites in broiler hatchlings[J]. Acta Scientiarum Animal Sciences, 2020, 42:e46029.doi: 10.4025/actascianimsci.v42i1.46029. doi: 10.4025/actascianimsci.v42i1.46029 URL
[20]	Harsij M, Gholipour Kanani H, Adineh H. Effects of antioxidant supplementation(nano-selenium,vitamin C and E)on growth performance,blood biochemistry,immune status and body composition of rainbow trout(Oncorhynchus mykiss)under sub-lethal ammonia exposure[J]. Aquaculture, 2020, 521:734942.doi: 10.1016/j.aquaculture.2020.734942. doi: 10.1016/j.aquaculture.2020.734942 URL
[21]	Markovic' R, C'iric' J, Starcˇevic' M, Šefer D, Baltic' M Ž. Effects of selenium source and level in diet on glutathione peroxidase activity,tissue selenium distribution,and growth performance in poultry[J]. Animal Health Research Reviews, 2018, 19(2):166-176.doi: 10.1017/S1466252318000105. doi: 10.1017/S1466252318000105 URL
[22]	侍苗苗. 纳米硒对中华绒螯蟹生长性能和抗氧化能力的影响研究[D]. 苏州: 苏州大学, 2015.
	Shi M M. Effects of dietary nano-se on the growth performance and oxidation resistance for Eriocheir sinensis[D]. Suzhou: Soochow University, 2015.
[23]	Köhrl J, Brigelius-Flohé R, Böck A, Gärtner R, Meyer O, Floh L. Selenium in biology:Facts and medical perspectives[J]. Biological Chemistry, 2000, 381(9/10):849-864.doi: 10.1515/BC.2000.107. doi: 10.1515/BC.2000.107
[24]	Neamat-Allah A N F, Mahmoud E A, Abd El Hakim Y. Efficacy of dietary Nano-selenium on growth,immune response,antioxidant,transcriptomic profile and resistance of Nile tilapia,Oreochromis niloticus against Streptococcus iniae infection[J]. Fish & Shellfish Immunology, 2019, 94:280-287.doi: 10.1016/j.fsi.2019.09.019. doi: 10.1016/j.fsi.2019.09.019
[25]	姜旭阳, 黄铭, 杨小刚, 周演根, 高勤峰, 董双林. 急性高温胁迫对虹鳟和硬头鳟幼鱼抗氧化酶活性的影响[J]. 中国水产科学, 2021, 28(1):57-65.doi: 10.12264/JFSC2020-0138. doi: 10.12264/JFSC2020-0138
	Jiang X Y, Huang M, Yang X G, Zhou Y G, Gao Q F, Dong S L. Antioxidant enzyme activities of juvenile rainbow and steelhead trout(Oncorhynchus mykiss)in response to acute high-temperature stress[J]. Journal of Fishery Sciences of China, 2021, 28(1):57-65.
[26]	Lledías F, Rangel P, Hansberg W. Oxidation of catalase by singlet oxygen[J]. Journal of Biological Chemistry, 1998, 273(17):10630-10637.doi: 10.1074/jbc.273.17.10630. doi: 10.1074/jbc.273.17.10630 pmid: 9553125
[27]	何婕. 高温和热浪对倭蛙热生物学、运动性能、氧化应激和抗氧化防御的影响[D]. 兰州: 兰州大学, 2021.doi: 10.27204/d.cnki.glzhu.2021.000645. doi: 10.27204/d.cnki.glzhu.2021.000645
	He J. Effects of high temperature and heat wave on thermal biology,locomotor performance,oxidative stress and antioxidant defense in Nanorana pleskei[D]. Lanzhou: Lanzhou University, 2021.
[28]	王红权, 唐德约, 赵玉蓉, 王小娜, 金柏涛, 张凯, 肖调义. 牛膝多糖对草鱼免疫和抗氧化功能的影响[J]. 水生生物学报, 2013, 37(2):351-357.doi: 10.7541/2013.26. doi: 10.7541/2013.26
	Wang H Q, Tang D Y, Zhao Y R, Wang X N, Jin B T, Zhang K, Xiao D Y. Effects of dietary Achyranthes bidentata polysaccharide on immunity and antioxidant function of grass carp Ctenopharyngodon idellus[J]. Acta Hydrobiologica Sinica, 2013, 37(2):351-357.
[29]	石璐璐, 王哲奇, 徐元庆, 毛晨羽, 郭世伟, 金晓, 史彬林. 热应激对绵羊血清免疫和抗氧化指标及相关基因相对表达量的影响[J]. 动物营养学报, 2020, 32(11):5275-5284.doi: 10.3969/j.issn.1006-267x.2020.11.032. doi: 10.3969/j.issn.1006-267x.2020.11.032
	Shi L L, Wang Z Q, Xu Y Q, Mao C Y, Guo S W, Jin X, Shi B L. Effects of heat stress on serum immune and antioxidative indexes and relative expression of related genes in sheep[J]. Chinese Journal of Animal Nutrition, 2020, 32(11):5275-5284.
[30]	Shi L L, Xu Y Q, Mao C Y, Wang Z Q, Guo S W, Jin X, Yan S M, Shi B L. Effects of heat stress on antioxidant status and immune function and expression of related genes in lambs[J]. International Journal of Biometeorology, 2020, 64(12):2093-2104.doi: 10.1007/s00484-020-02000-0. doi: 10.1007/s00484-020-02000-0 URL
[31]	Keyt B A, Baliga R, Sinclair A M, Carroll S F, Peterson M S. Structure,function,and therapeutic use of IgM antibodies[J]. Antibodies, 2020, 9(4):53.doi: 10.3390/antib9040053. doi: 10.3390/antib9040053 URL
[32]	Choi Y J, Kim N N, Shin H S, Park M S, Kil G S, Choi C Y. Effects of waterborne selenium exposure on the antioxidant and immunological activity in the goldfish,Carassius auratus[J]. Molecular & Cellular Toxicology, 2013, 9(4):365-373.doi: 10.1007/s13273-013-0045-9. doi: 10.1007/s13273-013-0045-9
[33]	管敏, 张德志, 唐大明. 慢性氨氮胁迫对史氏鲟幼鱼生长及其肝脏抗氧化、免疫指标的影响[J]. 南方水产科学, 2020, 16(2):36-42.doi: 10.12131/20190191. doi: 10.12131/20190191
	Guan M, Zhang D Z, Tang D M. Effects of chronic ammonia stress on growth,antioxidative and immunity indices in liver of juvenile Acipenser schrenckii[J]. South China Fisheries Science, 2020, 16(2):36-42.
[34]	刘飞, 王凡, 李聪歌, 马雅雯, 朱荣帅, 黄琼波. 红枣提取物对虹鳟血清指标和头肾免疫相关基因表达的影响[J]. 淡水渔业, 2020, 50(1):15-21.doi: 10.13721/j.cnki.dsyy.2020.01.003. doi: 10.13721/j.cnki.dsyy.2020.01.003
	Liu F, Wang F, Li C G, Ma Y W, Zhu R S, Huang Q B. Effects of jujube extracts on serum biochemical indexes and immune-related gene expression in the head kidney of rainbow trout(Oncorhynchus mykiss)[J]. Freshwater Fisheries, 2020, 50(1):15-21.
[35]	刘波. 高温应激与大黄蒽醌提取物对团头鲂生理反应及相关应激蛋白表达的影响[D]. 南京: 南京农业大学, 2012.
	Liu B. Effects of high temperature stress and anthraquinone extract from Rheum officinale bail on the physiological response and gene expression of stress heat shock protein of Wuchang bream(Megalobrama amblycephala)[D]. Nanjing: Nanjing Agricultural University, 2012.
[36]	陈星灿, 王磊, 相兴伟, 周宇芳, 邵庆均, 郑斌, 肖金星. 不同类型硒对黑鲷幼鱼生长及血清免疫指标的影响[J]. 水产科学, 2018, 37(5):577-583.doi: 10.16378/j.cnki.1003-1111.2018.05.001. doi: 10.16378/j.cnki.1003-1111.2018.05.001
	Chen X C, Wang L, Xiang X W, Zhou Y F, Shao Q J, Zheng B, Xiao J X. Effect of dietary selenium sources on growth performance and serum immune indices of juvenile black seabream,Acanthopagrus schlegelii[J]. Fisheries Science, 2018, 37(5):577-583.

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