摘要： 以虹鳟鱼(Oncorhynchus mykiss)为研究材料,使用RT-PCR和RACE法分离和克隆了虹鳟鱼NDK-1基因cDNA的全长序列(GenBank登录号:(FJ607868).序列全长953 bp,其中5'端非翻译区(5'-UTR)长5 bp,3'端非翻译区(3'-UTR)长28 bp,开放性阅读框(ORF)长62 bp,编码207个氨基酸.虹鳟鱼NDPK蛋白序列与几种脊椎动物台湾樱花钩吻鲑、斑马鱼、西方爪蟾、非洲爪蟾、人和黑青斑河的同源性分别为95%,72%,68%,68%,65% 和 6%,表明NDK-1基因在进化过程中是高度保守的.
关键词:
虹鳟鱼,
NDK-1 基因,
RT-PCR,
RACE
Abstract: RT-PCR and RACE (Rapid Amplification of cDNA Ends)methods were used for the isolation of the fulllength cDNA of NDK-1 gene (GenBank accession number :FJ591154)from rainbow trout.Sequence analysis revealed a 953 bp cDNA containing the 45 bp 5′-UTR, 284 bp 3′-UTR and 624 bp ORF encoding 207 amino acids.Sequence alignment of NDPK amino acid between rainbow trout and salmon, zebrafish,western frog, africa frog, human and fugu exhibited 95 %, 72 %, 68 %, 68 %, 65 % and 64 %identity rate, respectively.The result indicated the NDK-1 gene was highly conservative in the progress of evolution.
Key words:
words:Oncorhynchus mykiss,
NDK-1 gene,
RT-PCR,
RACE
中图分类号:
王家庆, 刘晓慧, 李代宗, 韩进诚, 郭冉, 王岳鸿, 王志平. 虹鳟鱼NDK-1 基因cDNA的克隆与序列特征分析[J]. 华北农学报, 2009, 24(4): 70-73. doi: 10.7668/hbnxb.2009.04.014.
WANG Jia-qing, LIU Xiao-hui, LI Dai-zong, HAN Jin-cheng, GUO Ran, WANG Yue-hong, WANG Zhi-ping,. The Cloning and Analysis of NDK-1 cDNA Derived from Rainbow Trout (Oncorhynchus mykiss)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 70-73. doi: 10.7668/hbnxb.2009.04.014.